A Novel Species Description of Sphingobium agrisoil sp. nov

Two Gram-stain positive, motile, aerobic, yellowish rod-shaped bacterial strains sgz301303 and sgz301304^T were isolated from the paddy soils. The 16S rRNA gene phylogenetic analysis revealed that both strains belong to the genus Sphingobium, and exhibited the highest sequence similarity (98.3%) with reference strain Sphingobium amiense JCM 11777^T. The draft genome of sgz301304^T has a size of 4.3 Mbp, with DNA G + C content of 64.5%. Whole-genome relatedness analyses showed that the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain sgz301304^T and S. amiense JCM 11777^T were 82.3% and 25.7%, respectively, both below the accepted species delineation thresholds. The main quinone was Q-10, and the main fatty acids included C_14:0 2-OH (11.3%), and Summed Features 8 (46.6%). Phylogenetic, genomic and phenotypic analyses suggest that strain sgz301304^T belongs to a novel species within the genus of Sphingobium. Therefore, it is recommended to be named as Sphingobium agrisoil sp. nov. The type strain is sgz301304^T (= MCCC 1K09180^T = JCM 37704^T), respectively.