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Updated: Feb 25, 2026

Innervation of Human Intestinal Organoids

Innervation of Human Intestinal Organoids

Published on: January 17, 2025

Single Cell-Seeded Human Intestinal Organoids for Organoid Research.

A Young Sim¹, Ha Young Kim¹, Gil Young Jung¹

¹Department of Anatomy, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine.

Journal of Visualized Experiments : Jove

|February 23, 2026

Summary

This summary is machine-generated.

A new method using single cell-seeded patient-derived intestinal organoids (PDIOs) significantly reduces variability in organoid size and number. This standardization enhances experimental consistency and reproducibility for organoid research.

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Last Updated: Feb 25, 2026

Innervation of Human Intestinal Organoids

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Published on: January 17, 2025

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights

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Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

Published on: July 29, 2021

Area of Science:

Biotechnology
Gastroenterology
Regenerative Medicine

Background:

Patient-derived intestinal organoids (PDIOs) model human gut tissue but exhibit variability.
Conventional culture methods lead to inconsistent organoid size and number, impacting experimental reliability.

Purpose of the Study:

To develop a standardized method for generating PDIOs with reduced heterogeneity.
To improve the reproducibility of experiments using PDIOs.

Main Methods:

Mature PDIOs were enzymatically dissociated into single cells.
Single cells were seeded at a defined number into 96-well plates.
Organoid growth and variability were assessed and compared to conventional methods.

Main Results:

Single cell-seeding normalized PDIO size and number, reducing inter-well and intra-well variability.
This approach improved the reproducibility of viability assays.
The method is compatible with downstream transcriptomic and proteomic analyses.

Conclusions:

Single cell-seeded PDIOs offer a standardized platform for reproducible organoid studies.
This method enhances experimental consistency for PDIO-based research.
It provides a practical foundation for advancing gut organoid research and applications.