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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Updated: Feb 25, 2026

Modified Methods for Loading of High-Throughput DNA Extraction Plates Reduce Potential for Contamination

Modified Methods for Loading of High-Throughput DNA Extraction Plates Reduce Potential for Contamination

Published on: June 3, 2020

Automated High-Throughput Proteomics Sample Preparation Platform Using DNA Extraction Plate for Industrial

Zuoqing Zhang^1,2, Manman Han¹, Yujie Wang¹

¹Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Systems Biology Centre, Technical Support Core Facilities, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.

Analytical Chemistry

|February 23, 2026

Summary

This summary is machine-generated.

We developed an automated proteomics sample preparation workflow (AutoDEP) for industrial microorganisms, significantly improving efficiency and reproducibility. This high-throughput method accelerates cell factory design and systems biology.

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Last Updated: Feb 25, 2026

Modified Methods for Loading of High-Throughput DNA Extraction Plates Reduce Potential for Contamination

Modified Methods for Loading of High-Throughput DNA Extraction Plates Reduce Potential for Contamination

Published on: June 3, 2020

Digital Microfluidics for Automated Proteomic Processing

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

Published on: September 27, 2016

Area of Science:

Microbiology
Proteomics
Biotechnology

Background:

Proteomic analysis is crucial for understanding microbial systems and designing cellular factories.
Current methods are manual, time-consuming, costly, and prone to errors.

Purpose of the Study:

To develop an automated, high-throughput proteomics sample preparation workflow (AutoDEP) for industrial microorganisms.
To improve efficiency, reduce errors, and accelerate proteomic data generation.

Main Methods:

Integration of a sodium dodecyl sulfate (SDS)-based lysis buffer with a 96-well DNA extraction plate.
Utilized the Biomek i7 liquid handling system for full automation from cell lysis to peptide generation.

Main Results:

AutoDEP achieved excellent sample preparation efficiency with over 81% zero missed cleavage rate.
High reproducibility was demonstrated with intraplate CV median below 7% and interplate CVs below 20% for over 91% of proteins.
Interday reproducibility showed a Pearson correlation coefficient (R²) exceeding 0.98.
The workflow robustly handles protein loading amounts from 5 to 200 μg.

Conclusions:

AutoDEP significantly accelerates the construction of cell models using industrial chassis cells.
The workflow's robust performance indicates potential for large-scale studies, including clinical sample analysis.