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Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

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Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
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Catenins01:23

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Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
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Anaphase Promoting Complex00:50

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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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Structural Protein Function01:56

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Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
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Structure of Cadherins01:25

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The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
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Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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Related Experiment Video

Updated: Feb 25, 2026

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
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Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

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DCN-type NEDD8 E3 ligases: Structure, biological function and small-molecule inhibitor.

Wenjuan Zhou1, Chenhao Xu2, Shengnan Zhang1

  • 1Children's Hospital Affiliated to Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450018, China.

Pharmacological Research
|February 23, 2026
PubMed
Summary
This summary is machine-generated.

Defective in cullin neddylation 1-5 (DCN1-5) enzymes are crucial for protein modification and disease. This review highlights DCN1-5 inhibitors as promising therapeutic targets for cancer and NRF2-related diseases.

Keywords:
Biological functionDCN1-5E3 ligasesInhibitorNeddylationStructure

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Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
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Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

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Area of Science:

  • Biochemistry and Molecular Biology
  • Medicinal Chemistry
  • Oncology

Background:

  • Defective in cullin neddylation 1-5 (DCN1-5) are essential E3 ligases in the neddylation pathway.
  • They catalyze cullin neddylation, activating Cullin-RING Ligases (CRLs) and modulating protein activity.
  • DCN1-5 play significant roles in cancer, fibrotic diseases, and other human pathologies.

Purpose of the Study:

  • To review the structure and biological functions of DCN1-5 enzymes.
  • To emphasize medicinal chemistry advancements in developing DCN1-5 inhibitors.
  • To discuss DCN1-5 as therapeutic targets for human diseases.

Main Methods:

  • Literature review of DCN1-5 structure, function, and biological roles.
  • Analysis of medicinal chemistry efforts in DCN1 inhibitor development.
  • Discussion of therapeutic potential in various disease contexts.

Main Results:

  • DCN1-5 are key regulators of CRLs, impacting numerous cellular processes.
  • Various chemotypes targeting DCN1 have been developed and evaluated.
  • DCN1 inhibitors show promise in preclinical studies for cancer and NRF2-related diseases.

Conclusions:

  • DCN1-5 are validated therapeutic targets due to their roles in disease pathogenesis.
  • Medicinal chemistry has made significant progress in developing DCN1 inhibitors.
  • Targeting DCN1-5 offers a promising strategy for treating cancer and other human diseases.