Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.7K
Proteomics01:33

Proteomics

10.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Radiolabeled Mesoporous Silica Nanosystem Enables Long-Term Cell Tracking In Vivo.

Molecular pharmaceutics·2026
Same author

Integrative single-cell and spatial transcriptomic analysis reveals a lactate-driven crosstalk between NFATc4⁺ tumor cells and SPP1⁺ macrophages in glioblastoma.

Journal of translational medicine·2026
Same author

Efficacy of limaprost combined with unilateral biportal endoscopic surgery in the treatment of lumbar spinal stenosis: based on time effects and stratified analysis.

Frontiers in surgery·2026
Same author

Publisher Correction: Spatial transcriptomics uncovers vasculature-centered cellular interactions driving Japanese encephalitis progression in a mouse model.

Nature communications·2026
Same author

SpatioTemporal Omics Consortium: a global effort for biological discovery across species, space and time.

Nature methods·2026
Same author

Targeting the Gut Microbiota with Herbal Compounds from Traditional Chinese Medicine: A Mechanistic Synthesis of a Novel Therapeutic Approach for Ulcerative Colitis.

Journal of inflammation research·2026

Related Experiment Video

Updated: Feb 25, 2026

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

14.6K

Nanopore-based massively parallel sensing for peptide profiling and protein identification.

Ji Wang1, Junyi Chen1, Hailin Pan1

  • 1State Key Laboratory of Genome and Multi-Omics Technologies, BGI Research, Shenzhen, China.

Nature Communications
|February 23, 2026
PubMed
Summary

We developed a high-throughput nanopore sensing platform using AI for accurate peptide and protein identification. This method streamlines analysis, enabling rapid antibody validation and advancing proteomics research.

More Related Videos

Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

13.1K
Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

10.4K

Related Experiment Videos

Last Updated: Feb 25, 2026

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

14.6K
Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

13.1K
Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

10.4K

Area of Science:

  • Biotechnology
  • Proteomics
  • Analytical Chemistry

Background:

  • Nanopore sensing offers potential for single-molecule proteomics but faces limitations in throughput and data analysis.
  • Current methods struggle with complex signals from stochastic events, hindering practical applications.

Purpose of the Study:

  • To develop a high-throughput nanopore sensing platform for enhanced proteomics.
  • To enable accurate peptide differentiation and protein identification using AI-driven analysis.
  • To establish a rapid workflow for antibody validation and epitope screening.

Main Methods:

  • Coupling a streamlined peptide library preparation with an AI-driven analytical workflow.
  • Capturing statistical signatures from single-molecule event streams for peptide fingerprinting.
  • Applying the platform for antibody validation, epitope screening, and protein identification from enzymatic digests.

Main Results:

  • Achieved remarkable classification accuracy for peptides through AI-driven analysis of event streams.
  • Demonstrated a rapid, cost-effective workflow for antibody validation and semi-quantitative affinity determination.
  • Successfully identified multiple proteins from complex enzymatic digests in a blinded study, showcasing system robustness.

Conclusions:

  • Developed a scalable and powerful end-to-end pipeline for proteomics research, from sample modification to identification.
  • The AI-driven nanopore platform overcomes previous limitations, offering high throughput and analytical power.
  • This method significantly advances single-molecule proteomics and antibody characterization.