A high-quality Chromosome-level genome assembly of Gynostemma guangxiense (Cucurbitaceae)

Gynostemma guangxiense X. X. Chen & D. H. Qin, belonging to the family Cucurbitaceae, is a perennial creeping herbaceous plant endemic to China with potential medicinal and health value. Here, we report the high-quality chromosome-level genome of G. guangxiense obtained by integrating Illumina short read, PacBio high-fidelity (HiFi) long read, Hi-C, and RNA-Seq technologies. The genome is anchored to 11 pseudochromosomes with a total size of 565.18 Mb, with a scaffold N50 of 52.63 Mb, achieving a complete BUSCO of 98.00%. Furthermore, we identified 27,527 protein-coding genes, of which 97.75% were functionally annotated. This genome provides an important molecular foundation for adaptive evolution, genetic conservation, and effective development of valuable medicinal plant resources within the Gynostemma genus.