Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

10.1K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.1K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

16.5K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
16.5K
T Cell Types and Functions01:24

T Cell Types and Functions

2.8K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
2.8K
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

3.4K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.4K
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

9.8K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
9.8K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.9K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Repurposing BH3 mimetics to deplete tumour-infiltrating regulatory T cells and enhance anti-tumour immunity in non-small cell lung cancer.

Cell death and differentiation·2026
Same author

Conserved T cell receptor usage underpins recognition of CD1c presenting a mycobacterial lipid.

bioRxiv : the preprint server for biology·2026
Same author

Control of poorly immunogenic tumors with systemic STING agonist-loaded liposomes targeting cross-presenting dendritic cells.

Clinical & translational immunology·2026
Same author

BCL-2 and MCL1 co-inhibition enhances glucocorticoid therapy in preclinical models of infant KMT2A-rearranged acute lymphoblastic leukaemia.

British journal of haematology·2026
Same author

Identification of T:B Cell Multimers After Bispecific T Cell Engagement, Using Both Conventional and Imaging Flow Cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same author

Parity, body mass index, smoking and risk of rheumatoid arthritis: data from the Australian Longitudinal Study on Women's Health.

BMC rheumatology·2026

Related Experiment Video

Updated: Feb 25, 2026

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice
07:34

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

Published on: December 16, 2021

3.3K

The NF-κB transcription factor RelA directs mucosal-associated invariant T-cell development.

Thomas S Fulford1,2, Hui-Fern Koay1, Raelene Grumont2

  • 1Department of Microbiology & Immunology at the Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, VIC, Australia.

Immunology and Cell Biology
|February 24, 2026
PubMed
Summary

Linear ubiquitin signaling complex (LUBAC) and NF-κB signaling are vital for mucosal-associated invariant T (MAIT) cell development in the thymus. These pathways are crucial for establishing the MAIT cell pool, impacting immune responses.

Keywords:
LUBACMAIT cellsNF‐κBRelAdevelopment

More Related Videos

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
08:37

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

Published on: February 15, 2021

4.9K
Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
08:37

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Published on: April 21, 2015

17.6K

Related Experiment Videos

Last Updated: Feb 25, 2026

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice
07:34

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

Published on: December 16, 2021

3.3K
Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
08:37

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

Published on: February 15, 2021

4.9K
Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
08:37

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Published on: April 21, 2015

17.6K

Area of Science:

  • Immunology
  • Cell Biology
  • Transcription Factors

Background:

  • Mucosal-associated invariant T (MAIT) cells are crucial immune cells with rapid responses to nonpeptide antigens.
  • The transcription factor PLZF is known to be essential for MAIT cell function, but developmental pathways remain unclear.

Purpose of the Study:

  • To investigate the transcriptional programs governing MAIT cell development in the thymus.
  • To identify key signaling pathways and transcription factors involved in MAIT cell differentiation.

Main Methods:

  • Analysis of MAIT cell development in genetically modified mouse models lacking specific NF-κB pathway components and LUBAC.
  • Assessment of MAIT cell thymic development and antigen responsiveness.

Main Results:

  • RelA, a canonical NF-κB transcription factor, is critical for MAIT cell thymic development but not antigen response.
  • NF-κB1 and c-Rel have minor roles in MAIT cell development.
  • MAIT cell development is significantly impaired in the absence of LUBAC, an upstream regulator of NF-κB signaling.

Conclusions:

  • LUBAC and NF-κB signaling pathways play essential roles in the transcriptional network controlling MAIT cell development.
  • These findings elucidate critical regulators of MAIT cell pool establishment, impacting future immunotherapies.