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Updated: Jun 1, 2026

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

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Published on: May 21, 2015

Intra-Tissue Bacteriome and Cellular Profiles in Periodontal Granulation Tissue From Osseous Defects and Extraction

Tianfan Cheng¹, Tianle Li^2,3, Tsz Yung Wong¹

¹Division of Periodontology & Implant Dentistry, Faculty of Dentistry, The University of Hong Kong, Hong Kong, SAR, China.

Journal of Clinical Periodontology

|February 24, 2026

Summary

Periodontal granulation tissue (PGT) shows a healthier microbiome and more mesenchymal stem cells (MSCs) than diseased tissues. This suggests PGT could be a source for periodontal regeneration therapies.

Keywords:

bacteriome granulation tissue periodontal defect periodontitis single‐cell RNA‐sequencing

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Area of Science:

Periodontology
Microbiology
Cell Biology

Background:

Periodontal disease involves complex interactions between bacteria and host cells.
Periodontal granulation tissue (PGT) is a byproduct of chronic inflammation and healing.

Purpose of the Study:

To characterize the bacteriome and cellular composition of PGT.
To explore the potential of PGT for periodontal regeneration.

Main Methods:

Collected PGT samples from 49 severe periodontitis patients.
Utilized 16S rRNA sequencing, single-cell sequencing, and histology.
Compared PGT with diseased tissues (pocket wall, extraction sockets, root surfaces).

Main Results:

PGT showed a commensal-enriched bacteriome, unlike periodontopathogen-enriched diseased tissues.
PGT had more mesenchymal stem cells (MSCs) and fibroblasts, fewer natural killer cells.
PGT exhibited reduced inflammation and immune cell infiltration compared to diseased tissues.

Conclusions:

PGT possesses periodontal health-associated characteristics.
PGT is a potential source of MSCs for periodontal regeneration.