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Draft genome sequence of an entomopathogenic fungus Amphichorda felina
Ngoc-Hung Nguyen1,2, Phuong-Thao Nguyen2, Toshimitsu Fukiharu3
1Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan.
Abstract:
An entomopathogenic fungus, Amphichorda felina (formerly classified as Beauveria felina), was sequenced through Illumina MiSeq short reads and Oxford Nanopore long reads. The hybrid assembly resulted in a genome of approximately 29.6 Mb with 9 scaffolds and an N50 of 3.28 Mb.
Insights
The genome of the entomopathogenic fungus Amphichorda felina was sequenced using advanced methods. This resulted in a high-quality genome assembly, providing valuable resources for fungal research.
Area of Science:
- Mycology
- Genomics
- Insect Pathology
Background:
- Amphichorda felina is an entomopathogenic fungus.
- Accurate classification and genomic data are crucial for understanding fungal biology and applications.
Purpose of the Study:
- To sequence and assemble the genome of Amphichorda felina.
- To provide a high-quality genomic resource for this entomopathogenic fungus.
Main Methods:
- Whole-genome sequencing using Illumina MiSeq (short reads) and Oxford Nanopore (long reads).
- Hybrid genome assembly approach.
Main Results:
- A genome size of approximately 29.6 Mb was achieved.
- The assembly comprised 9 scaffolds with an N50 value of 3.28 Mb.
Conclusions:
- The successful hybrid assembly provides a robust genome for Amphichorda felina.
- This genomic resource will facilitate future research into fungal genetics, evolution, and biocontrol potential.
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