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Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food
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Sequential attachment and Listeria predominance under turbulent flow
Krisha Pant1, Jon Palmer1, Steve Flint1
1School of Natural Sciences and Food Technology, Massey University, Palmerston North, New Zealand.
Biofouling
|February 24, 2026
Summary
Sequential colonization significantly impacts multispecies biofilm formation under turbulent flow. This study highlights how the order of bacterial attachment, specifically Listeria monocytogenes and Pseudomonas fluorescens, influences biofilm development and structure.
Area of Science:
- Microbiology
- Environmental Science
- Biotechnology
Background:
- Multispecies biofilms are crucial for understanding bacterial interactions in natural and industrial settings.
- Traditional studies often assume simultaneous bacterial colonization, overlooking sequential attachment dynamics.
- Sequential colonization is a critical factor in multispecies biofilm formation, yet often unexamined.
Purpose of the Study:
- To investigate the impact of sequential colonization on multispecies biofilm formation.
- To compare biofilm development under turbulent flow versus static conditions.
- To analyze the roles of Pseudomonas fluorescens and Listeria monocytogenes in sequential biofilm assembly.
Main Methods:
- Studied sequential and co-colonization of Pseudomonas fluorescens and Listeria monocytogenes on stainless steel.
- Assessed biofilm formation under turbulent flow and static conditions.
- Quantified bacterial attachment and cell concentration over time.
Main Results:
- Under turbulent flow, Listeria monocytogenes attachment was significantly higher on preformed Pseudomonas fluorescens biofilms compared to sterile surfaces or co-inoculation.
- Listeria monocytogenes reached peak cell concentration earlier (24h) on established Pseudomonas fluorescens biofilms.
- Exopolysaccharides from Pseudomonas fluorescens did not influence Listeria monocytogenes attachment, emphasizing biofilm architecture's role.
Conclusions:
- The order of bacterial colonization is a key determinant of multispecies biofilm formation, particularly under turbulent flow conditions.
- Sequential colonization dynamics are essential variables for studying bacterial community lifestyles in biofilms.
- Understanding sequential attachment is vital for controlling and predicting biofilm development in industrial and environmental contexts.
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