Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Modification02:32

Histone Modification

16.6K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
16.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling.

Science advances·2026
Same author

The Development of New SSR Markers and an Assay for Genotyping Sweet Cherry (<i>Prunus avium</i> L.) in One Reaction.

International journal of molecular sciences·2026
Same author

Introgression of barley chromosome arms 4H and 6H into wheat via Robertsonian translocations: GBS-assisted structural analysis and impact on grain nutrient composition.

Plant molecular biology·2026
Same author

Sorghum embryos undergoing B chromosome elimination express B-variants of mitotic-related genes.

Genome biology·2025
Same author

Combined action of suicide gene exosomes from pancreatic cancer-associated fibroblasts and from mesenchymal stem cells as a pancreatic ductal adenocarcinoma treatment approach.

Cancer cell international·2025
Same author

Thermal Plasticity of Multiple Traits Varies More Within Than Between Populations of <i>Plantago lanceolata</i> at Its Northern Range Edge.

Ecology and evolution·2025
Same journal

A sugar flow model predicts cell dynamics, weight and quality of tomato at varying sink-source ratios and temperatures.

Journal of experimental botany·2026
Same journal

Crosstalks between plant proteostasis and chromatin remodeling machineries.

Journal of experimental botany·2026
Same journal

Novel Imaging Approaches for Visualising Root-Mycorrhizal Fungal Interactions.

Journal of experimental botany·2026
Same journal

The ga3ox1b mutation reveals the crosstalk between gibberellin and other phytohormones in controlling the growth and development of female flowers in Cucurbita pepo.

Journal of experimental botany·2026
Same journal

Increased grain weight conferred by GW2 mutations in wheat does not translate into yield gains in multi-year field trials of near-isogenic lines.

Journal of experimental botany·2026
Same journal

Serendipita indica promotes rice phosphorus uptake by plasma membrane H+-ATPase OsA1-stimulated root hair growth.

Journal of experimental botany·2026
See all related articles

Related Experiment Video

Updated: Feb 26, 2026

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
07:10

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Published on: March 4, 2021

5.1K

Enhanced plant bottom-up histone proteomics.

Palina Ryzhaya1,2, Pavlína Pírek1, Radomír Pech1

  • 1Mendel Center for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno 625 00, Czech Republic.

Journal of Experimental Botany
|February 24, 2026
PubMed
Summary
This summary is machine-generated.

This study enhances plant histone analysis using mass spectrometry (MS) by optimizing sample preparation. New workflows yield pure histone extracts for detailed epigenetic studies in crops.

Keywords:
Zea maysCrop plantsflow cytometryhistone derivatizationmaizemass spectrometrypost-translational modificationproteomicstrimethylacetic anhydride

More Related Videos

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Published on: May 17, 2016

30.6K
Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
09:48

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Published on: April 22, 2022

4.5K

Related Experiment Videos

Last Updated: Feb 26, 2026

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
07:10

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Published on: March 4, 2021

5.1K
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Published on: May 17, 2016

30.6K
Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
09:48

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Published on: April 22, 2022

4.5K

Area of Science:

  • Plant Biology
  • Proteomics
  • Epigenetics

Background:

  • Histone proteoform characterization is crucial for understanding plant epigenetics and biotechnology.
  • Bottom-up proteomics offers a way to study the epigenetic landscape of plant chromatin.
  • Mass spectrometry (MS) analysis of histone peptides requires careful sample preparation, including chemical derivatization, for optimal nanoHPLC separation.

Purpose of the Study:

  • To evaluate different histone preparation protocols from Zea mays leaves for improved mass spectrometry (MS) data quality.
  • To enhance existing MS-based plant histone analysis protocols for better characterization of histone proteoforms.

Main Methods:

  • Optimized histone extraction from Zea mays leaves.
  • Chemical derivatization of amine groups using trimethylacetic anhydride.
  • Enzymatic digestion utilizing a novel protease, Arg-C Ultra.
  • Fluorescence-assisted cell sorting (FACS) for pure histone sample isolation.

Main Results:

  • Developed an enhanced protocol combining chemical derivatization and enzymatic digestion for plant histone analysis.
  • Demonstrated the effectiveness of FACS in obtaining pure histone samples, bypassing traditional precipitation methods.
  • Achieved highly pure histone extracts suitable for quantitative analysis of post-translational modifications and variant composition.

Conclusions:

  • The proposed workflows significantly improve the quality of mass spectrometry data for plant histone analysis.
  • These methods provide a powerful tool for investigating epigenetic patterns and dynamics in agriculturally important crops.
  • Enhanced histone characterization facilitates advancements in plant biotechnology and crop improvement.