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Updated: Feb 26, 2026

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Structural Insight Into Jasmonic Acid Signalling Repression by Insect HARP1 Effector.

Yaguang Zhang1, Baoyu He2, Tingting Ran3

  • 1State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.

Plant, Cell & Environment
|February 24, 2026
PubMed
Summary
This summary is machine-generated.

The insect effector HARP1 binds to plant JAZ proteins, blocking jasmonic acid (JA) signaling. This structural study reveals how pests suppress plant defenses by inhibiting JA pathway activation.

Keywords:
HARP1JA signallingJAZcrystal structuredimerisation

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Area of Science:

  • Plant-insect interactions
  • Molecular plant-pathology
  • Biochemistry

Background:

  • Plants and pests engage in co-evolutionary arms races.
  • Pest effectors, like HARP1 from Helicoverpa armigera, can suppress plant immunity.
  • Jasmonic acid (JA) signaling is crucial for plant defense against biotic stress.

Purpose of the Study:

  • To elucidate the mechanism by which HARP1 suppresses JA signaling.
  • To identify the JAZ domain responsible for HARP1 interaction.
  • To provide structural insights into HARP1-JAZ binding.

Main Methods:

  • Biochemical assays to confirm HARP1-JAZ interaction domain.
  • X-ray crystallography to determine HARP1 structure.
  • AlphaFold2 prediction for HARP1-JAZ3 binding mode analysis.

Main Results:

  • The ZIM domain of JAZ is sufficient for HARP1 binding.
  • HARP1 possesses a seven-stranded β-sandwich fold.
  • HARP1 directly binds JAZ dimers, inhibiting JAZ degradation and repressing JA signaling.

Conclusions:

  • HARP1 binding to JAZ proteins is a key mechanism for pest suppression of plant defenses.
  • Structural and functional data reveal how pest effectors disrupt plant JA signaling.
  • This study offers insights into transcriptional repression of JA signaling by insect-derived effectors.