Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Myocarditis I: Introduction

Myocarditis I: Introduction

Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

TRAF6 Lactylation in Glycolytic Macrophages Drives NF-κB Signaling and M1 Polarization During Orthodontic Tooth Movement.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Development of a serum-based microRNA panel for Alzheimer's disease diagnosis.

Journal of translational internal medicine·2026

Same author

Functional Characterisation of NF-YCs in True Leaf Biomass Accumulation.

Plants (Basel, Switzerland)·2026

Same author

Effects of letrozole supplementation on growth performance, plasma hormones, and plasma metabolites in weaned female lambs of Turpan black sheep.

Frontiers in veterinary science·2026

Same author

The effects of supplementing Astragalus and fermented Astragalus on lactation performance, rumen microbiota, and lamb weight gain in Turpan black sheep.

Frontiers in microbiology·2026

Same author

Effects of whole cotton seed on plasma metabolism and lactation performance of sheep.

Tropical animal health and production·2026

Search research articles

Related Experiment Video

Updated: Feb 26, 2026

A Microscopic 2,3,5-Triphenyltetrazolium Chloride Assay for Accurate and Reliable Analysis of Myocardial Injury

A Microscopic 2,3,5-Triphenyltetrazolium Chloride Assay for Accurate and Reliable Analysis of Myocardial Injury

Published on: November 28, 2025

PAF Triggered Pyroptotic NETosis Aggravates Myocardial Ischemia/Reperfusion Injury.

Jiawei Wu^1,2, Shule Zhang^1,3, Ruofan Du^1,3

¹State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, P. R. China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|February 25, 2026

Summary

Platelet activating factor (PAF) drives neutrophil extracellular trap (NET) formation, worsening myocardial ischemia-reperfusion (MI/R) injury. Dapagliflozin inhibits this process, offering a potential therapeutic strategy for MI/R injury.

Keywords:

NETosis dapagliflozin myocardial ischemia‐reperfusion injury platelet activating factor

More Related Videos

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Published on: March 7, 2022

Author Spotlight: A Novel Standardized Technique for Real-Time Biomedical Imaging of Acute Myocardial Injury

Author Spotlight: A Novel Standardized Technique for Real-Time Biomedical Imaging of Acute Myocardial Injury

Published on: March 22, 2024

Related Experiment Videos

Last Updated: Feb 26, 2026

A Microscopic 2,3,5-Triphenyltetrazolium Chloride Assay for Accurate and Reliable Analysis of Myocardial Injury

A Microscopic 2,3,5-Triphenyltetrazolium Chloride Assay for Accurate and Reliable Analysis of Myocardial Injury

Published on: November 28, 2025

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Published on: March 7, 2022

Author Spotlight: A Novel Standardized Technique for Real-Time Biomedical Imaging of Acute Myocardial Injury

Author Spotlight: A Novel Standardized Technique for Real-Time Biomedical Imaging of Acute Myocardial Injury

Published on: March 22, 2024

Area of Science:

Cardiovascular Research
Immunology
Metabolic Signaling

Background:

Myocardial ischemia-reperfusion (MI/R) injury is a significant clinical problem.
The interplay between metabolic and inflammatory pathways in MI/R injury is not fully understood.

Purpose of the Study:

To investigate the role of neutrophil extracellular traps (NETs) and platelet activating factor (PAF) in MI/R injury.
To identify therapeutic targets for mitigating MI/R injury.

Main Methods:

Verified NETosis as a key driver of MI/R injury.
Demonstrated PAF secreted by cardiomyocytes induces NETosis via PLA2G6 and gasdermin D (GSDMD).
Evaluated dapagliflozin as a NETosis inhibitor targeting lipocalin-2 (LCN2) in a SGLT2-independent manner.

Main Results:

Inhibiting NETs or PAF synthesis significantly reduced MI/R injury in mice.
Dapagliflozin protected mice from MI/R injury.
Elevated serum PAF correlated with NETosis and myocardial injury in patients; dapagliflozin use was associated with attenuated injury.

Conclusions:

PAF acts as a danger signal initiating NETosis in early MI/R injury.
Targeting the PAF-NETosis pathway with agents like dapagliflozin or LCN2 inhibitors shows therapeutic potential for MI/R injury.