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Published on: December 18, 2013
Exercise affects salivary biomarkers of creatine metabolism in healthy adults
Bogdan Andjelic1,2, Tijana Lainovic3, Nikola Todorovic1
1Applied Bioenergetics Lab, Faculty of Sport and Physical Education, University of Novi Sad, Novi Sad, Serbia.
Background:
We monitored changes in salivary creatine pre- and post-high-intensity exercise in young adults while also investigating the potential correlation between salivary and serum creatine levels.
Method:
Saliva and serum samples were collected before and immediately after an incremental running-to-exhaustion treadmill test in fifteen young adults (mean age [23.9 ± 2.9] years, eight females), with samples analyzed for guanidinoacetic acid, creatine, and creatinine using a liquid chromatography-tandem mass spectrometry method.
Results:
Following exercise, there was a substantial elevation in salivary creatine levels from (17.5 ± 14.2) μmol·L-1 to (43.6 ± 30.4) μmol·L-1 (p < 0.001), coupled with a significant increase in salivary creatinine from (11.3 ± 5.8) μmol·L-1 to (17.0 ± 9.3) μmol·L-1 (p = 0.04). In contrast, serum creatine levels were unaffected by exercise (p = 0.80), while creatinine levels exhibited a strong tendency to decrease post-exercise (from [81.8 ± 17.5] μmol·L-1 to [73.1 ± 11.6] μmol·L-1; p = 0.06). A comparison of the slopes of the two regression lines (saliva vs. serum) revealed significant differences for both creatine (p = 0.01) and creatinine (p = 0.03).
Conclusions:
The above findings suggest a potential difference in the dynamics of creatine metabolites in these two bodily fluids, both pre and post-exercise.
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