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IR Spectroscopy: Molecular Vibration Overview01:24

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When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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A Multimodal Wide-Field Fourier-Transform Raman Microscope
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Far-field single-molecule vibrational spectroscopy and imaging.

Philip A Kocheril1, Haomin Wang1, Ryan E Leighton1

  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology Pasadena CA 91125 USA lwei@caltech.edu.

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Single-molecule vibrational spectroscopy now achieves far-field imaging under ambient conditions. This breakthrough offers unprecedented insights into molecular dynamics and behavior at the single-molecule level.

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Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Nanotechnology

Background:

  • Single-molecule (SM) optical spectroscopy provides deep insights into molecular dynamics.
  • Vibrational spectro-microscopies traditionally face challenges with weak signals.
  • Recent advancements enable far-field vibrational spectroscopy and imaging at the SM level.

Purpose of the Study:

  • To review critical insights and advancements enabling SM vibrational spectroscopy and imaging.
  • To highlight technical developments overcoming the SM barrier with far-field optics.
  • To discuss the opportunities presented by SM vibrational methods.

Main Methods:

  • Far-field optical techniques.
  • Advanced vibrational spectroscopy.
  • Single-molecule imaging and analysis.

Main Results:

  • Realization of far-field vibrational spectroscopy at the single-molecule level.
  • Overcoming signal limitations in vibrational spectro-microscopies.
  • Demonstration of SM vibrational imaging under ambient conditions.

Conclusions:

  • SM vibrational spectroscopy and imaging are now feasible under ambient conditions.
  • Technical progress has enabled breaking the SM barrier using far-field optics.
  • These methods open exciting opportunities for studying molecular behavior.