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Related Experiment Video

Updated: Jul 26, 2026

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape
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Pure Chitosan Microfluidic Spinning Affords Modular Core-Sheath Fibers and Hand-Crafted Scaffolds with Enhanced

Ishneet Kaur1, Alejandro Forigua2, Hatem M Titi1

  • 1Centre for Green Chemistry and Catalysis, Department of Chemistry, McGill University, Montréal, Quebec, Canada.

Small (Weinheim an Der Bergstrasse, Germany)
|February 25, 2026
PubMed
Summary

Researchers developed pure chitosan fibers using microfluidic wet spinning for tissue engineering. These novel biomaterial fibers offer superior strength and cell viability compared to alginate, with potential for complex tissue regeneration.

Keywords:
biocompatibilitychitosan microfibershydrogel fibersmicrofluidic wet spinningoxidized chitin nanocrystals

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Hydrogel fibers are crucial for tissue engineering, mimicking in vivo structures like blood vessels.
  • Alginates are common but have poor cell adhesion and require crosslinking, leading to ion leaching and instability.
  • Limitations of current alginate-based fibers necessitate advanced biomaterials for improved tissue constructs.

Purpose of the Study:

  • To synthesize pure chitosan fibers via microfluidic wet spinning, overcoming alginate limitations.
  • To evaluate the cell viability, mechanical properties, and processability of chitosan fibers.
  • To explore the incorporation of chitin nanocrystals for enhanced functionality.

Main Methods:

  • Microfluidic wet spinning technique for fabricating pure chitosan fibers.
  • Assessment of cell viability using standard biological assays.
  • Mechanical testing to compare chitosan fibers with alginate counterparts.
  • Incorporation of chitin nanocrystals into the chitosan matrix.

Main Results:

  • Achieved excellent cell viability (85%) in pure chitosan fibers.
  • Demonstrated significantly higher mechanical strength (695 MPa) compared to alginate fibers (2-4 MPa).
  • Chitosan fibers showed high processability and potential for creating composite structures with chitin nanocrystals.

Conclusions:

  • Pure chitosan fibers produced by microfluidic wet spinning offer a superior alternative to alginate for tissue engineering.
  • These fibers provide enhanced mechanical strength, excellent cell viability, and versatile functionality.
  • The developed chitosan and chitin/chitosan fibers hold significant potential for fabricating complex artificial tissues and regenerative medicine applications.