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iChip

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

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Related Experiment Video

Updated: Jun 23, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

MiProChip: A Scalable Microfluidic Platform for Multiplexed Single-Cell Proteomics via Isobaric Labeling.

Tsai-Fang Chou¹, Huan-Chi Chiu^1,2, Sofani Tafesse Gebreyesus¹

¹Institute of Chemistry, Academia Sinica, Taipei 11529, Taiwan.

Analytical Chemistry

|February 25, 2026

Summary

This summary is machine-generated.

MiProChip is a new microfluidic platform for single-cell proteomics (SCP) that streamlines workflows and enhances proteome coverage. This advanced tool captures subtle cellular responses to treatments, offering deeper insights than bulk analysis.

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Published on: November 15, 2017

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

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Last Updated: Jun 23, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

Area of Science:

Biomedical Engineering
Proteomics
Microfluidics

Background:

Single-cell proteomics (SCP) is crucial for understanding cellular heterogeneity.
Existing SCP platforms require optimization for throughput and sensitivity.
Microfluidic technologies offer potential for streamlined omics workflows.

Purpose of the Study:

To develop and validate MiProChip, an integrated microfluidic platform for multiplexed single-cell proteomics.
To optimize on-chip tandem mass tag (TMT) labeling for high-throughput SCP.
To demonstrate MiProChip's capability in characterizing cellular responses to stimuli.

Main Methods:

Development of MiProChip integrating cell capture, lysis, digestion, TMT labeling, pooling, and desalting.
Optimization of a chip-compatible TMT multiplexing protocol with carrier-boosting.
Application of MiProChip to analyze murine colon adenocarcinoma cells treated with galectin-8 and TGF-β.

Main Results:

MiProChip enabled identification of 3362 protein groups across TMT-10-plex channels using PC9 and H1975 cells.
Analysis of murine colon cancer cells identified 3199 proteins, with 1669 proteins per cell.
Single-cell PCA revealed distinct cellular responses to galectin-8 and TGF-β, with TGF-β showing a dominant effect.
Pathway analysis identified specific responses and revealed galectin-8's antimetastatic potential, undetected by bulk analysis.

Conclusions:

MiProChip provides a sensitive and robust platform for high-throughput single-cell proteomics.
The platform effectively captures subtle proteomic heterogeneity and treatment-dependent responses at the single-cell level.
MiProChip offers advantages over bulk proteomic analysis for uncovering specific cellular mechanisms.