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Related Concept Videos

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Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Updated: Feb 28, 2026

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
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An Integrative Proteotranscriptomics Approach Reveals New ADAM9 Substrates and Downstream Pathways.

Congyu Lu1, Xiaolu Xu2, Neha Sindhu1

  • 1Department of Biological Sciences, University of Delaware, Newark, Delaware, USA; Center for Bioinformatics and Computational Biology, University of Delaware, Newark, Delaware, USA.

Molecular & Cellular Proteomics : MCP
|February 25, 2026
PubMed
Summary
This summary is machine-generated.

This study identifies targets of the metalloprotease ADAM9, revealing its role in key cancer pathways like mTOR and FOXO. This work offers new insights into ADAM9

Keywords:
ADAM9FOXOectodomain sheddingmTORproteotranscriptomics

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • ADAM9 is a cell-surface metalloprotease involved in ectodomain shedding.
  • Dysregulated ADAM9 activity is linked to diseases including cancer, autoimmunity, and COVID-19.
  • Understanding ADAM9 substrates is crucial for elucidating its roles in health and disease.

Purpose of the Study:

  • To systematically identify transcriptional and post-transcriptional targets of ADAM9.
  • To uncover signaling pathways regulated by ADAM9.
  • To identify direct and indirect substrates of ADAM9.

Main Methods:

  • Integrative proteotranscriptomics approach.
  • Utilized HCT116 cells with a stable diploid karyotype for omics analyses.
  • Systematic identification of ADAM9 targets.

Main Results:

  • Identified major signaling pathways downstream of ADAM9, including the mTOR and FOXO pathways.
  • Uncovered direct and indirect substrates of ADAM9.
  • Provided mechanistic insights into ADAM9 function.

Conclusions:

  • ADAM9 regulates key oncogenic (mTOR) and tumor suppressor (FOXO) pathways.
  • Identified novel substrates mediating ADAM9's pathophysiological roles.
  • Developed a transferable method for studying membrane proteases.