In-planta Agrobacterium-mediated cereal transformation-InPACT
Nirman Nepal1, Fariba Rafiei1, Steve Scofield2
1Department of Agronomy, Purdue University, West Lafayette, IN, United States.
Plant Physiology
|February 25, 2026
Summary
A new method, In-planta Agrobacterium-mediated cereal transformation (InPACT), enables efficient and genotype-flexible genetic modification in small grain cereals like wheat, barley, oats, and rice.
Area of Science:
- Plant Science
- Biotechnology
- Genetics
Background:
- Efficient genetic transformation is crucial for crop improvement in cereals.
- Existing methods often lack genotype flexibility and efficiency, hindering progress.
Purpose of the Study:
- To develop a simple, efficient, and genotype-flexible method for cereal transformation.
- To demonstrate the broad applicability of the new method across diverse cereal species and genotypes.
Main Methods:
- The In-planta Agrobacterium-mediated cereal transformation (InPACT) method was developed.
- InPACT was tested on 32 wheat genotypes (29 hexaploid, 3 tetraploid), along with barley, oat, and rice germplasm.
Main Results:
- Successful transformation was achieved across 32 wheat genotypes, showcasing significant genotype flexibility.
- The InPACT method proved highly reproducible, with both experienced users and learners achieving successful transgenic wheat generation.
- The method's utility was confirmed in other cereal crops, including barley, oat, and rice.
Conclusions:
- InPACT offers a robust and versatile platform for genetic engineering in small grain cereals.
- This method has the potential to accelerate crop breeding and research in wheat, barley, oats, and rice.
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