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Long non-coding RNAs in response to Ebola virus vaccine-induced immunity
Izabela Mamede1, Thomaz Luschër-Dias2, Isabelle Franco Moscardini3
1Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Frontiers in Immunology
|February 26, 2026
Summary
Long noncoding RNAs (lncRNAs) are key regulators of gene expression. This study reveals conserved lncRNA activation after Ebola vaccination, suggesting their role in human immune responses.
Area of Science:
- Immunology
- Genetics
- Molecular Biology
Background:
- Long noncoding RNAs (lncRNAs) regulate gene expression but their role in human vaccine responses is unknown.
- Understanding lncRNA dynamics is crucial for optimizing vaccine efficacy.
Purpose of the Study:
- To investigate lncRNA expression patterns following vaccination in humans.
- To identify specific lncRNAs involved in immune responses to the Ebola vaccine.
Main Methods:
- Analysis of RNA-sequencing data from three independent human cohorts vaccinated with the rVSVΔG-ZEBOV-GP Ebola vaccine.
- Differential expression analysis and correlation meta-analysis were performed.
- Integration with global run-on sequencing and comparative transcriptomic analysis.
Main Results:
- Identified a conserved lncRNA expression signature, including LEF1-AS1 and DOCK8-AS1, activated post-vaccination.
- Correlated lncRNA expression with gene targets and IgG titers, indicating roles in innate and adaptive immunity.
- Functional enrichment revealed involvement in T-cell differentiation, interferon signaling, and leukocyte activation.
Conclusions:
- lncRNAs are integral components of the human vaccine response.
- LEF1-AS1 modulation is distinctively associated with Ebola vaccination.
- Findings provide a basis for future studies on noncoding RNA regulation of immunity.
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