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Related Concept Videos

Ischemic Stroke l: Introduction01:15

Ischemic Stroke l: Introduction

Ischemic stroke is an acute cerebrovascular condition in which blood flow to a brain region is suddenly interrupted, leading to tissue infarction. Neurons depend on continuous oxygen and glucose supply, so even brief reductions in perfusion cause energy failure, ionic imbalance, and irreversible injury. Ischemic strokes are classified into thrombotic and embolic types based on their underlying mechanisms.Thrombotic MechanismsThrombotic stroke develops when a clot forms within a cerebral artery.
Ischemic Stroke ll: Pathophysiology01:15

Ischemic Stroke ll: Pathophysiology

An ischemic stroke occurs when a cerebral blood vessel becomes obstructed, most often by a thrombus or embolus, interrupting the delivery of oxygen and glucose to brain tissue. Because neurons rely on continuous aerobic metabolism, energy failure begins within minutes of reduced perfusion. The region receiving the least blood flow becomes the infarct core, an area of irreversible cellular death. Surrounding this core lies the penumbra, a zone of hypoperfused but still viable tissue that is...

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Quantification of Neurovascular Protection Following Repetitive Hypoxic Preconditioning and Transient Middle Cerebral Artery Occlusion in Mice
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CD177 Expression on Neutrophils Predicts Ischemic Stroke Outcome in Humans.

Janine Gronewold1, Nina Hagemann1, Yiqiao Zhang1

  • 1Department of Neurology (J.G., N.H., Y.Z., M.F., A.M.Y., A.L., C.W., E.D., B.F., A.M., A.G., C.G., E.M.N., M.d.S.R., M.N., D.M.H.), University Hospital Essen, University of Duisburg-Essen, Germany.

Stroke
|February 26, 2026
PubMed
Summary

Individuals lacking the CD177 protein (CD177-deficient) experienced worse outcomes after ischemic stroke compared to those with CD177 (CD177 wild-type). CD177 expression levels also predict stroke severity and clinical outcomes.

Keywords:
flow cytometryinnate immunityischemic strokeneutrophilsstrokestroke rehabilitationvalue of tests

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Area of Science:

  • Immunology
  • Neurology
  • Genetics

Background:

  • Neutrophils contribute to tissue damage following ischemic stroke.
  • CD177 is a membrane protein found on neutrophils, with a small percentage of the population lacking it (CD177-deficient genotype).
  • The role of CD177 in ischemic stroke is not well understood.

Purpose of the Study:

  • To investigate the impact of CD177 deficiency and expression levels on stroke severity and patient outcomes.
  • To evaluate CD177 as a potential biomarker for stroke stratification and as a therapeutic target.

Main Methods:

  • Prospective analysis of two patient cohorts (PROSCIS-B and NOFF-S) with first-ever ischemic stroke.
  • Assessment of CD177 status (null vs. wild-type) and expression levels (neg, dim, high) using flow cytometry.
  • Evaluation of stroke severity and outcomes using the National Institutes of Health Stroke Scale and modified Rankin Scale, analyzed with multivariable regression and discrimination analyses.

Main Results:

  • Patients with CD177 deficiency (CD177null) showed a significantly higher probability of unfavorable stroke outcomes compared to CD177 wild-type (CD177WT) individuals.
  • CD177null status independently predicted worse stroke outcomes after adjusting for clinical factors.
  • Higher CD177dim neutrophil proportions correlated with lower stroke severity, while CD177high neutrophils predicted favorable outcomes.
  • CD177 expression levels significantly improved the prediction of stroke outcomes beyond established clinical variables.

Conclusions:

  • CD177 expression serves as an easily measurable biomarker for stratifying stroke patients.
  • CD177 presents a potential therapeutic target for modulating immune responses in the context of stroke.