Multi-stage assembled Lactobacillus reuteri-capsule patch for sustained antibacterial therapy

Area of Science:

• Biomaterials Science
• Microbiology
• Regenerative Medicine

Background:

• Recurrent bacterial infections impede wound healing.
• Developing sustained antibacterial strategies is crucial for managing infected sites.
• Traditional antibiotics can lead to resistance and inflammation.

Purpose of the Study:

• To develop a sustained antibacterial therapeutic strategy using a Lactobacillus reuteri-capsule patch.
• To evaluate the patch's efficacy in preventing recurrent bacterial invasion and promoting tissue regeneration.

Main Methods:

• A multi-stage assembly strategy was used to create a Lactobacillus reuteri-encapsulated sodium alginate (SA) and bacterial cellulose (BC) nanofiber patch.
• The patch's sustained release, antibacterial activity against biofilms (E. coli, S. aureus, MRSA, P. aeruginosa), and bioactivities were assessed.
• In vivo infection models were used to verify the patch's effectiveness in clearing biofilms and promoting tissue repair.

Main Results:

• The encapsulated Lactobacillus reuteri demonstrated sustained release and antibacterial activity, inhibiting biofilm formation by 99.9%.
• Transcriptomics revealed the patch promotes proliferation, migration, and vascularization, unlike traditional antibiotics.
• In vivo studies confirmed biofilm clearance, prevention of recurrent Pseudomonas aeruginosa invasion, and enhanced tissue regeneration via collagen deposition and neovascularization.

Conclusions:

• The Lactobacillus reuteri-capsule patch provides a simple, efficient approach for persistent anti-infection applications.
• This strategy effectively combats recurrent bacterial invasion and promotes wound healing through tissue regeneration.
• The patch represents a promising alternative to traditional antibiotic therapies for infected sites.