Isolation, Identification, and Management Strategies for the Root Rot Pathogen of Cardamine violifolia.

Area of Science:

• Plant Pathology
• Agricultural Science
• Mycology

Background:

• Root rot disease significantly reduces Cardamine violifolia's yield.
• Causal pathogens and control strategies for Cardamine violifolia root rot are poorly understood.

Purpose of the Study:

• To identify key pathogens causing root rot in Cardamine violifolia.
• To develop and validate an effective fungicide for disease control.

Main Methods:

• Isolation and identification of pathogens using morphological and phylogenetic analyses.
• Pathogenicity testing and fungicide screening via plate inhibition assays.
• Optimization and validation of fungicide combinations through soil drenching experiments.

Main Results:

• Aspergillus costaricensis, Mucor circinelloides cf. lusitanicus, and Fusarium pernambucanum were identified as key pathogens.
• A novel ternary fungicide (T10) combining propiconazole, hymexazol, and difenoconazole demonstrated significantly enhanced potency.
• T10 exhibited superior EC50 values and increased inhibition rates against all tested pathogens compared to single fungicide applications.

Conclusions:

• The identified pathogens are responsible for Cardamine violifolia root rot.
• The novel ternary fungicide T10 offers a potent and effective solution for managing this disease.
• This research provides a foundation for sustainable disease management in selenium-enriched crops.