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Non-invasive Optical Measurement of Cerebral Metabolism and Hemodynamics in Infants
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Evaluation of Probe Positioning Effects on Optical Parameters in Neonatal Forehead Time-Resolved Spectroscopy

Yoko Tadatomo1,2, Kota Inoue1, Tomohito Nakayama3

  • 1Department of Pediatrics, Faculty of Medicine, Kagawa University, Takamatsu 760-0016, Kagawa, Japan.

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|February 26, 2026
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Summary

The midline forehead is optimal for neonatal cerebral monitoring using time-resolved spectroscopy (TRS). While key measures like total hemoglobin and oxygen saturation were consistent, the midline offered more stable scattering and optical path data for reliable monitoring.

Keywords:
neonateprobe positioningtime-resolved spectroscopy

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Area of Science:

  • Medical Physics
  • Neonatal Physiology
  • Biomedical Optics

Background:

  • Time-resolved spectroscopy (TRS) offers noninvasive cerebral monitoring for neonates.
  • Optimal probe placement for TRS in neonates requires further investigation.
  • Understanding optical parameter variations with probe site is crucial for accurate neonatal monitoring.

Purpose of the Study:

  • To evaluate the impact of probe positioning on TRS-derived optical parameters in neonates.
  • To compare measurements from the midline and lateral forehead sites.
  • To determine the most suitable forehead location for neonatal cerebral monitoring using TRS.

Main Methods:

  • TRS measurements were conducted on 30 neonates (≥36 weeks' corrected gestational age).
  • Data was collected from both the midline and right lateral forehead.
  • Key optical parameters including intensity, attenuation, path length, scattering, total hemoglobin (tHb), cerebral oxygen saturation (ScO2), and cerebral blood volume (CBV) were analyzed.

Main Results:

  • No significant differences in tHb, ScO2, or CBV were found between midline and lateral sites.
  • The lateral site exhibited a significantly lower scattering coefficient and shorter mean optical path length.
  • Increased light intensity and reduced attenuation were observed at the lateral site compared to the midline.

Conclusions:

  • While physiological parameters (tHb, ScO2, CBV) are consistent across sites, the midline forehead provides more stable scattering and optical path length data.
  • The midline forehead is suggested as a more suitable site for reliable TRS-based neonatal cerebral monitoring.
  • These findings can optimize TRS probe placement protocols for neonatal intensive care units.