Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.6K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PLK1: A Promising Therapeutic Target for Prostate Cancer Treatment.

Serican journal of medicine·2026
Same author

Correction: Molecular mechanisms of α-syn abnormal phase separation in cognitive impairment induced by chronic intermittent hypoxia and the neuroprotective effects of Danshensu methyl ester.

Molecular medicine (Cambridge, Mass.)·2026
Same author

Efficient Scheduling of Heterogeneous Messages in the FlexRay Dynamic Segment.

Sensors (Basel, Switzerland)·2026
Same author

ESAK-YOLO: multi-scale attention enhanced object detection for floating waste in complex water scenes.

Scientific reports·2026
Same author

DNA Tetrahedron-Mediated Sensing Platform on a Single Gold Nanowire: Special Signal Amplification, Self-Referencing Capability, and Cross-Validated Exosome Detection for Liver Cancer Liquid Biopsy.

ACS sensors·2026
Same author

The role of endogenous hormones in regulating grain number in oat panicles under drought stress across cultivars.

Frontiers in plant science·2026

Related Experiment Video

Updated: Feb 27, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.3K

Polystyrene Microsphere-Labeled Lateral Flow Assay for the Visual Detection of Foodborne Pathogens.

Lingmei Zhang1,2, Wanwei Qiu1, Lu Lu1

  • 1School of Biomedicine and Health, Anhui Science and Technology University, Chuzhou 233100, China.

Biosensors
|February 26, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a new polystyrene microsphere-based lateral flow assay (LFA) for detecting Staphylococcus aureus. This method offers improved sensitivity and stability for rapid foodborne pathogen detection compared to traditional gold nanoparticle LFAs.

Keywords:
PCR productsfood safetylateral flow assaypolystyrene microspheres

More Related Videos

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
12:50

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

Published on: June 9, 2014

15.1K
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

14.4K

Related Experiment Videos

Last Updated: Feb 27, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.3K
Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
12:50

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

Published on: June 9, 2014

15.1K
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

14.4K

Area of Science:

  • Food Safety
  • Analytical Chemistry
  • Biotechnology

Background:

  • Growing demand for rapid, sensitive, and cost-effective foodborne pathogen detection methods.
  • Limitations of traditional gold nanoparticle-labeled lateral flow assays (LFAs) in signal strength for nucleic acid detection.

Purpose of the Study:

  • To develop an improved LFA using polystyrene microspheres for detecting Staphylococcus aureus nucleic acids.
  • To enhance probe stability and signal strength compared to gold nanoparticle-based LFAs.

Main Methods:

  • Construction of a polystyrene microsphere-labeled LFA targeting the nuc gene of Staphylococcus aureus.
  • Utilizing a covalent coupling strategy for enhanced probe attachment.
  • Detection of double-stranded PCR products.

Main Results:

  • Achieved a detection limit of 7.28 × 10^2 CFU/mL, a tenfold improvement over gold nanoparticle LFAs.
  • Demonstrated excellent specificity, reproducibility (RSD < 5%), and stability.
  • Polystyrene microsphere LFAs showed superior performance in artificially contaminated milk samples.

Conclusions:

  • Polystyrene microsphere-labeled LFAs provide a more sensitive and stable platform for detecting foodborne pathogens.
  • This method offers an efficient and user-friendly solution for visual detection of Staphylococcus aureus.
  • The developed assay has significant potential for point-of-care testing in food safety applications.