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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Related Experiment Video

Updated: Feb 27, 2026

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

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Published on: September 10, 2016

A New Single-Chain, Genetically Encoded Biosensor for RhoB GTPase Based on FRET, Useful for Live-Cell Imaging.

Sandra Pagano¹, Louis Hodgson^1,2

¹Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

|February 26, 2026

Summary

This summary is machine-generated.

Researchers developed a new FRET biosensor to visualize RhoB GTPase activity in living cells. This tool reveals RhoB

Keywords:

FRET RhoGTPase biosensor endosome

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Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

RhoB GTPase is crucial for membrane trafficking and cytoskeletal regulation.
Its function depends on subcellular localization, but tools to monitor its activity are limited.

Purpose of the Study:

To develop and validate a genetically encoded Förster resonance energy transfer (FRET) biosensor for RhoB.
To visualize RhoB activity in real-time within living cells.

Main Methods:

Development of a single-chain FRET biosensor for RhoB.
Live-cell imaging of RhoB activity in migrating mouse embryonic fibroblasts.
Multiplex imaging with a TC10 GTPase biosensor.

Main Results:

The RhoB biosensor visualizes spatially heterogeneous activity during cell migration.
Coordinated yet antagonistic patterns of RhoB and TC10 activities were observed at the leading edge.
TC10 regulation impacts the spatial coupling of RhoB and TC10 activities.

Conclusions:

A novel and robust FRET biosensor for RhoB has been created.
A multiplex imaging framework allows studying the coordination of RhoB and TC10 in cell migration.
This work advances the understanding of Rho GTPase signaling in cellular processes.