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Separation of Avian Preovulatory Follicle Granulosa and Theca Cell Layers for Downstream Applications
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LncRNA RORB-IT1 Encoding a Micropeptide Regulates Progesterone Synthesis, Proliferation and Apoptosis in Chicken
Jie Cao1,2, Qingqing Wei1,2, Li Kang1,2
1College of Animal Science and Technology, Shandong Agricultural University, 7 Panhe Street, Taian 271017, China.
Cells
|February 26, 2026
Summary
RAR-related orphan receptor B-intronic transcript 1 (RORB-IT1), a novel long non-coding RNA, regulates chicken ovarian follicular development by influencing progesterone synthesis, cell proliferation, and apoptosis in granulosa cells, potentially improving egg-laying performance.
Area of Science:
- Animal Science
- Molecular Biology
- Reproductive Biology
Background:
- Ovarian follicular development is crucial for chicken egg-laying performance.
- Long non-coding RNAs (lncRNAs) are emerging regulators of follicular development.
- RAR-related orphan receptor B-intronic transcript 1 (RORB-IT1) was previously identified as differentially expressed in chicken granulosa cells.
Purpose of the Study:
- To characterize the expression pattern of RORB-IT1 in chicken ovarian follicular granulosa cells (Post-GCs).
- To investigate the role of RORB-IT1 in regulating progesterone synthesis, proliferation, and apoptosis of chicken Post-GCs.
- To explore the functional micropeptide encoded by RORB-IT1.
Main Methods:
- Quantitative analysis of RORB-IT1 expression in chicken Post-GCs.
- Hormonal stimulation assays (FSH, P4, E2) to assess RORB-IT1 regulation.
- Functional assays to evaluate RORB-IT1's impact on progesterone synthesis, proliferation, and apoptosis.
- Micropeptide identification and functional characterization.
Main Results:
- RORB-IT1 is specifically expressed in chicken Post-GCs and its expression is upregulated by FSH, P4, and E2.
- RORB-IT1 promotes progesterone synthesis and proliferation while inhibiting apoptosis in Post-GCs.
- RORB-IT1 encodes a micropeptide that enhances progesterone synthesis and proliferation but induces apoptosis when overexpressed independently.
Conclusions:
- RORB-IT1 plays a significant role in regulating chicken ovarian follicular development.
- RORB-IT1 influences key cellular processes including steroidogenesis, proliferation, and apoptosis in Post-GCs.
- Understanding RORB-IT1 function provides a theoretical basis for enhancing chicken egg-laying performance.
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