Ultrasound-Guided Mandibular Alveolar Nerve Block in Rabbits: A Cadaveric Comparison of In-Plane and Out-of-Plane
Matteo Serpieri1, Andrea Degiovanni1, Giuseppe Bonaffini1,2
1Department of Veterinary Sciences, University of Turin, Largo Braccini 2, 10095 Grugliasco, Italy.
Veterinary Sciences
|February 26, 2026
Summary
Ultrasound-guided mandibular alveolar nerve blocks in rabbits are anatomically feasible using either in-plane or out-of-plane approaches. This technique offers a potentially more accurate method for managing dental pain in rabbits.
Area of Science:
- Veterinary Medicine
- Anesthesiology
- Comparative Anatomy
Background:
- Mandibular dental disease is prevalent in pet rabbits, often necessitating invasive procedures with significant pain.
- Current blind techniques for mandibular alveolar nerve blocks lack objective accuracy evaluation.
- Ultrasound-guided approaches for this nerve block in rabbits have not been previously reported.
Purpose of the Study:
- To describe an ultrasound-guided mandibular alveolar nerve block technique in rabbits.
- To compare the efficacy of in-plane versus out-of-plane needle approaches.
- To assess the anatomical feasibility and accuracy of ultrasound guidance.
Main Methods:
- A cadaveric study involving twelve New Zealand White rabbits (24 nerves).
- One nerve per rabbit underwent an in-plane ultrasound-guided block, the contralateral used an out-of-plane approach.
- Injection of lidocaine and methylene blue, followed by assessment of image quality, staining, and dye spread.
Main Results:
- Both in-plane and out-of-plane approaches enabled consistent identification of the target region.
- Comparable ultrasound image quality, perineural staining scores, and longitudinal dye spread were observed between techniques.
- No statistically significant differences were found between the in-plane and out-of-plane approaches.
Conclusions:
- Ultrasound-guided mandibular alveolar nerve blocks are anatomically feasible in rabbits using either in-plane or out-of-plane techniques.
- The study demonstrates the potential for improved accuracy in regional anesthesia for rabbits.
- Further in vivo research is necessary to confirm clinical efficacy and safety.


