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Molecular Identification and Drug Sensitivity Test of Acinetobacter lwoffii from Cynomolgus Monkey with Peritonitis
Heling Li1, Ziyao Qian1, Lan Luo1
1State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China.
Abstract:
A case of peritonitis in a female cynomolgus monkey (Macaca fascicularis) caused by suspected bacterial infection was found. In order to identify the pathogenic bacteria, pathological tissues from the peritoneum of the animal were collected, and a bacterial strain designated MF080196 was isolated. Morphological observations were conducted, followed by biochemical testing and sequencing of its 16S rRNA gene. Additionally, pathogenicity test and drug sensitivity analyses were performed. The findings indicated that colonies grown on blood agar medium appeared white, circular, and raised; the bacteria were identified as Gram-negative; biochemical tests aligned with the characteristics of Acinetobacter lwoffii (A. lwoffii). PCR amplification was performed using a 16S rRNA sequence measuring 1443 bp; there was a remarkable similarity of 99.8% with previously reported sequences of A. lwoffii from various sources. The isolated strain MF080196 is capable of mortality in mice; furthermore, drug susceptibility testing revealed that the isolated strain exhibited high sensitivity to 23 antibiotics, including ceftriaxone, gentamicin, and levofloxacin. The pathogen isolated from the peritoneal tissue of the dead cynomolgus monkey was identified as A. lwoffii, which demonstrated sensitivity to most antibiotics tested in this study. This research provides significant insights for preventing and treating bacterial diseases in non-human primates.
Insights
Bacterial peritonitis in a cynomolgus monkey was caused by Acinetobacter lwoffii. This pathogen showed sensitivity to numerous antibiotics, offering insights for treating non-human primate infections.
Area of Science:
- Veterinary Microbiology
- Primate Pathology
Background:
- Peritonitis is a serious condition in non-human primates, often linked to bacterial infections.
- Identifying the causative agent is crucial for effective treatment and disease prevention in captive primates.
Purpose of the Study:
- To identify the bacterial pathogen responsible for peritonitis in a cynomolgus monkey.
- To characterize the isolated bacteria and assess its pathogenicity and antibiotic susceptibility.
Main Methods:
- Isolation and identification of bacteria from peritoneal tissues.
- Morphological, biochemical, and 16S rRNA gene sequencing for bacterial identification.
- Pathogenicity testing in mice and antibiotic susceptibility testing.
Main Results:
- A bacterial strain, MF080196, was isolated and identified as Acinetobacter lwoffii (A. lwoffii).
- The isolate showed 99.8% similarity to known A. lwoffii strains via 16S rRNA sequencing.
- MF080196 caused mortality in mice and was sensitive to 23 antibiotics, including ceftriaxone, gentamicin, and levofloxacin.
Conclusions:
- Acinetobacter lwoffii was identified as the causative agent of peritonitis in the cynomolgus monkey.
- The study highlights A. lwoffii's potential pathogenicity and its susceptibility to common antibiotics.
- Findings offer valuable information for managing bacterial infections in non-human primates.
