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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Related Experiment Video

Updated: Feb 27, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

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Deoxyribonucleic Acid Activator-Triggered Entropy-Driven Catalysis-Modulated CRISPR/Cas12a-Based Portable Biosensor

Shuai Liu1, Zishan Ding1, Xing Lu1

  • 1Department of Clinical Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, P. R. China.

ACS Sensors
|February 26, 2026
PubMed
Summary

A new portable biosensor rapidly detects multiple antibiotic-resistant bacteria (ARB) using CRISPR/Cas12a technology. This ultrasensitive platform offers a fast, cost-effective solution for clinical diagnostics and public health surveillance.

Keywords:
CRISPR/Cas12aantibiotic-resistant bacteriamicrofluidic chip devicemultiple detectionpersistent luminescent nanoparticles

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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

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Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Biosensing

Background:

  • Antibiotic-resistant bacteria (ARB) pose a significant global health threat.
  • Rapid and sensitive detection methods for ARB are crucial for effective clinical and public health interventions.
  • Current detection methods can be time-consuming and lack the sensitivity required for early identification.

Purpose of the Study:

  • To develop a portable, ultrasensitive, and rapid biosensor for the simultaneous detection of multiple ARB.
  • To enhance signal amplification and detection sensitivity using CRISPR/Cas12a and entropy-driven catalysis.
  • To integrate a 3D-printed microfluidic chip and smartphone readout for practical application.

Main Methods:

  • Construction of a portable DNA activator-triggered entropy-driven catalysis-modulated CRISPR/Cas12a-based sensor (PSDA).
  • Utilized CRISPR/Cas12a-mediated signal transduction with a target-specific DNA activator and an entropy-driven dynamic DNA network for signal amplification.
  • Integrated a 3D-printed microfluidic chip with Zn2GeO4:Mn persistent luminescent nanoparticles and a smartphone readout system.

Main Results:

  • The PSDA platform achieved ultrasensitive and rapid simultaneous detection of methicillin-resistant Staphylococcus aureus, carbapenem-resistant Pseudomonas aeruginosa, and KPC-2-expressing Klebsiella pneumoniae.
  • Demonstrated a broad dynamic range (1-10^7 CFU/mL) and an ultralow detection limit (1 CFU/mL).
  • Analysis time was approximately 45 minutes with high consistency compared to conventional methods (95.48-115.15%).

Conclusions:

  • The developed PSDA biosensing platform offers a cost-effective, rapid-response solution for simultaneous ARB detection.
  • This technology has direct applications in clinical diagnostics, food safety monitoring, and environmental surveillance.
  • The portable and sensitive nature of the sensor addresses the critical challenge of timely ARB identification.