Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

Chromatin Structure and RNA Splicing

Chromatin Structure and RNA Splicing

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Inheritance of Chromatin Structures

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Neuronal subtype-specific ribosomal protein mRNA expression.

RNA (New York, N.Y.)·2026

Same author

Single-cell RNA-seq reveals trans-sialidase-like superfamily gene expression heterogeneity in <i>Trypanosoma cruzi</i> populations.

eLife·2026

Same author

Application of Data-Centric Supervised Machine Learning to Predict Phenotypic Activity Against Clinically Relevant Stages of <i>Trypanosoma cruzi</i>.

Pharmaceutics·2025

Same author

Exploring Latin America one cell at a time.

Cell·2025

Same author

Comparative genomics sheds light on mammalian and avian gene regulation and phenotypic evolution.

Nature communications·2025

Same author

Exploring a gene co-expression network throughout the trypanosoma cruzi life cycle.

BMC genomics·2025

Same journal

Analysis of strength degradation of coal and rock masses and stability of mined areas under long term immersion environment.

PloS one·2026

Same journal

Biogenic Silver-Selenium nanocomposite with anticancer activity and potent efficacy against vancomycin-resistant Staphylococcus aureus.

PloS one·2026

Same journal

Preparation and physicochemical characterization of a biodegradable chitosan/carboxymethyl cellulose hydrogel synthesized in NaOH/urea medium.

PloS one·2026

Same journal

Action-guilt, survivor-guilt, and depression in combat-related PTSD.

PloS one·2026

Same journal

Explainable machine learning for predicting activities of daily living at discharge in stroke patients: A retrospective study using SHAP interpretability.

PloS one·2026

Same journal

Deep learning based two-way feature depiction model for brain tumor detection.

PloS one·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 28, 2026

Examination of the Telomere G-overhang Structure in Trypanosoma brucei

Examination of the Telomere G-overhang Structure in Trypanosoma brucei

Published on: January 26, 2011

Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids.

Romina Trinidad Zambrano Siri^1,2, Paula Beati¹, Lucas Inchausti^3,4

¹Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.

|February 26, 2026

Summary

This summary is machine-generated.

Researchers studied chromatin organization in TriTryps, identifying nucleosome depletion at trans-splicing acceptor sites (TASs). A protective complex, partly histone-based, shields TASs, suggesting a mechanism for accurate trans-splicing in these parasites.

More Related Videos

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Published on: October 17, 2019

Related Experiment Videos

Last Updated: Feb 28, 2026

Examination of the Telomere G-overhang Structure in Trypanosoma brucei

Examination of the Telomere G-overhang Structure in Trypanosoma brucei

Published on: January 26, 2011

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Published on: October 17, 2019

Area of Science:

Parasitology
Molecular Biology
Genetics

Background:

TriTryps (Trypanosoma cruzi, Trypanosoma brucei, Leishmania major) cause significant human and animal diseases.
These parasites have complex life cycles and unique gene expression mechanisms, including polycistronic transcription and trans-splicing.
Multi-copy genes are vital for TriTryp host invasion and immune evasion.

Purpose of the Study:

To predict trans-splicing acceptor sites (TASs) in TriTryps.
To investigate the chromatin organization surrounding TASs.
To explore potential mechanisms ensuring trans-splicing fidelity.

Main Methods:

Bioinformatic prediction of TASs.
Chromatin digestion and analysis (MNase sensitivity).
Comparative chromatin structure analysis between single and multi-copy genes.

Main Results:

Consistent chromatin organization across TriTryps with mild nucleosome depletion at TASs.
Identification of an MNase-sensitive complex, containing histones, protecting TASs in T. brucei.
Differential chromatin structure at TASs in T. cruzi single- versus multi-copy genes.

Conclusions:

Chromatin structure plays a role in regulating trans-splicing in TriTryps.
A histone-containing complex likely protects TASs, ensuring trans-splicing accuracy.
Differential chromatin organization may represent a novel mechanism for regulating gene expression fidelity in trypanosomatids.