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Updated: Feb 28, 2026
Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
Published on: July 10, 2014
Porcine-derived amelogenin P148 in a layer-by-layer chitosan/hyaluronic acid coating for enhanced implant
Chunbao Wang1, Pei Tao1, Yuzhao Cheng2
1School of Pharmacy, Yichun University, Yichun, Jiangxi 336000, China; State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Abstract:
Porcine-derived amelogenins, essential for enamel development and clinically applied in periodontal bone regeneration, faces purification challenges for its specific isoforms, limiting the understanding of their distinct functions and clinical translation. In this study, we purified P148, the most abundant isoform in porcine amelogenins, constructed a chitosan/hyaluronic acid (CS/HA) layer-by-layer coating on polydopamine-modified titanium implants, and preliminarily investigated its osteogenic effects and underlying mechanisms using in vitro and in vivo models. In vitro, the P148-functionalized surfaces exhibited favorable biocompatibility and significantly promoted cell attachment, proliferation, osteogenic gene expression, and extracellular matrix (ECM) production compared to unmodified titanium. Moreover, this modification activated the MAPK-ERK1/2 pathway, a key signaling axis regulating BMSC differentiation. In vivo rat experiments further verified enhanced peri-implant new bone formation, underscoring the translational promise of the P148/CS/HA coating for improving osseointegration. Taken together, these findings suggest that this bioactive surface holds broad application potential for clinical bone regeneration.
