Related Experiment Video

Updated: Feb 28, 2026

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

3.9K

SDC4 is a novel target of the KLF5 transcription factor

Yuhki Yokoyama1, Hiroyuki Yamamoto1, Akane Uomoto1

  • 1Department of Molecular Pathology, Division of Health Sciences, Graduate School of Medicine, The University of Osaka, 1-7, Yamadaoka, Suita, Osaka, 565-0871, Japan.

Biochemical and Biophysical Research Communications
|February 26, 2026
PubMed

Insights

The study reveals that the transcription factor KLF5 regulates the expression of SDC4, a protein linked to poor prognosis in gastrointestinal cancers. This finding offers new insights into cancer development and potential therapeutic targets.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Syndecan-4 (SDC4) is upregulated in gastrointestinal adenocarcinomas and associated with poor prognosis, tumor growth, and metastasis.
  • The regulatory mechanisms governing SDC4 gene expression in cancer remain largely unknown.

Purpose of the Study:

  • To investigate the transcriptional regulation of SDC4 in colorectal cancer.
  • To identify key transcription factors involved in controlling SDC4 expression.

Main Methods:

  • Bioinformatic analysis of the SDC4 promoter region for potential transcription factor binding sites.
  • Reporter gene assays in colorectal cancer cell lines to assess promoter activity.
  • Chromatin immunoprecipitation followed by quantitative PCR (ChIP-qPCR) to confirm protein-DNA interactions.
  • Immunohistochemical staining of human colorectal cancer tissues to correlate SDC4 and KLF5 expression.

Main Results:

  • Database analysis identified potential KLF5 binding sites within the SDC4 promoter.
  • Promoter assays highlighted two critical KLF5 binding regions (-70 to -40) essential for SDC4 promoter activity.
  • ChIP-qPCR confirmed direct binding of KLF5 to these critical promoter regions.
  • Immunohistochemistry revealed similar expression patterns of SDC4 and KLF5 in colorectal cancer tissues, with a significant positive correlation.

Conclusions:

  • SDC4 expression is transcriptionally regulated by KLF5 in colorectal cancer cells and tissues.
  • KLF5 is identified as a novel transcription factor targeting the SDC4 gene.
Keywords:
Colorectal cancerKLF5SDC4

More Related Videos

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins09:56

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins

Published on: October 31, 2025

519
A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

8.1K

Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.7K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.9K
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.8K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.7K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

3.1K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.2K