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Updated: Feb 28, 2026

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
Published on: August 4, 2018
Involvement of two ESCRT-Ⅲ accessory proteins, MoDid2 and MoVta1, in development, pathogenicity, and endocytosis in
Jian Liao1, Zi-Fang Shen2, Jing-Yi Wang3
1State Key Laboratory of Rice Biology and Breeding, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Abstract:
The endosomal sorting complex required for transport (ESCRT) is a highly conserved system for membrane remodeling in eukaryotes. The biological functions of ESCRT-Ⅲ accessory proteins, particularly in Magnaporthe oryzae, remain poorly understood, especially as they relate to the final step in cargo sorting. In this study, we identified the proteins Did2 and Vta1 in M. oryzae and elucidated their biological functions. Our findings indicate that MoDid2 and MoVta1 are predominantly localized to endosomes and play significant roles in vegetative growth, conidiation, and pathogenicity. Furthermore, MoDid2 and MoVta1 are involved in abiotic stress responses and influence endocytosis through their participation in the Oms1 MAPK and Pmk1 MAPK pathways, as well as modulating K63-linked ubiquitination levels. Additionally, MoDid2 affect autophagy but not MoVta1. In summary, our findings reveal a correlation between Did2/Vta1 and pathogenicity of M. oryzae, offering new insights for the control of rice blast disease.
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