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Published on: May 4, 2018
Efficient nitrogen removal lacking canonical genes: Decoding the unique nitrogen metabolic network in Providencia
Hui Zou1, Haiyan Wang1, Yansen Wei2
1Key Laboratory of Beijing on Regional Air Pollution Control, Department of Environmental Science, College of Environmental Science & Engineering, Beijing University of Technology, Beijing, 100124, China.
Abstract:
Heterotrophic nitrification-aerobic denitrification (HN-AD) is a crucial strategy for biological nitrogen removal in wastewater, yet its metabolic complexity remains poorly understood. This study reports a novel strain, Providencia manganoxydans AHY1, which performs efficient HN-AD despite lacking the canonical core genes (amo, hao, nirS/nirK, norBC, nosZ). Strain AHY1 achieved high-rate removal of NH4+-N (2.65 mg/L/h) and NO3--N (2.67 mg/L/h) with complete efficiency. Integrated multi-omics analysis decoded this exceptional capability, revealing a uniquely coordinated, non-canonical metabolic network. The core of this network lies in a putative Dirammox-like pathway inferred from genomic and transcriptomic evidence, an active hydroxylamine shunt, and a NorR-Hmp-dominated NO detoxification circuit, together with assimilatory/dissimilatory nitrate reduction and ammonium assimilation. Furthermore, strain AHY1 showed strong tolerance to multiple stressors, maintaining a 96.24% NH4+-N removal efficiency with 5 mg/L Ni2+ and 73.6% with 200 mg/L ampicillin. This study presents a novel mechanistic model of microbial nitrogen cycling and emphasizes the significant potential of strain AHY1 for treating complex wastewater lacking canonical nitrogen removal pathways.
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