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RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
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The Rice Cis-Natural Antisense Transcript NAT1850 of Pri-miR1850 Negatively Regulates Cold Tolerance by Repressing

Yang Shen1,2, Yan Wang1, Lijia Yang3

  • 1Crop Stress Molecular Biology Laboratory, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, China.

Plant Biotechnology Journal
|February 26, 2026
PubMed
Summary
This summary is machine-generated.

Natural antisense transcripts (NATs) and small interfering RNAs (siRNAs) regulate rice cold tolerance and yield. This study reveals a novel regulatory mechanism involving a pri-miRNA and its cis-NAT, impacting plant stress response and agricultural productivity.

Keywords:
NPR3miR1850siR1850cold tolerancegrain yieldnatural anti‐sense transcriptrice

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Area of Science:

  • Plant Molecular Biology
  • Genetics and Genomics
  • Agricultural Science

Background:

  • Natural antisense transcripts (NATs) are prevalent in rice but their functions remain largely uncharacterized.
  • Understanding NATs is crucial for improving rice resilience and yield.

Purpose of the Study:

  • To characterize a rice cis-NAT (NAT1850) overlapping with pri-miR1850.
  • To elucidate the regulatory roles of NAT1850 and its derived siRNA (siR1850) in rice cold tolerance and yield.

Main Methods:

  • Transcriptome analysis
  • RNA interference (RNAi) techniques
  • Gene expression analysis
  • Genetic studies

Main Results:

  • NAT1850 and siR1850 negatively regulate cold tolerance in rice.
  • siR1850 targets NPR3, a component of the cold stress signaling pathway involving WRKY76 and DREB1B.
  • NAT1850 and siR1850 also influence nitrogen assimilation and rice yield through independent pathways.

Conclusions:

  • A novel regulatory module involving a pri-miRNA and its cis-NAT (NAT1850-siR1850) is identified.
  • This module plays a dual role in balancing cold stress response and rice yield.
  • Findings provide insights into pri-miRNA regulation and its impact on plant development and stress adaptation.