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FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Updated: Jul 1, 2026

DNA-based Fish Species Identification Protocol

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

Recent insights for fish allergens: novel processing techniques and detection technology.

Hailin Yin¹, Xinyu Ge², Futeng Song¹

¹School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, China.

Journal of the Science of Food and Agriculture

|February 26, 2026

Summary

This summary is machine-generated.

Fish allergy is a major food safety concern. Innovative processing and detection methods, including artificial intelligence (AI), can reduce allergenicity and improve safety for fish products.

Keywords:

artificial intelligence combined processing methods detection technologies fish allergy next‐generation sequencing

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Last Updated: Jul 1, 2026

DNA-based Fish Species Identification Protocol

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Published on: April 28, 2010

Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin

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Published on: July 13, 2016

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Area of Science:

Food science
Allergy research
Biotechnology

Background:

Fish allergy is a prevalent food allergy impacting global food safety.
Understanding fish allergen properties and mechanisms is crucial for risk management.

Purpose of the Study:

To provide a comprehensive overview of fish allergy mechanisms and allergens.
To focus on innovative processing and detection technologies for fish allergy control.

Main Methods:

Combined processing: thermal treatment (glycation, irradiation), enzymatic hydrolysis with acid treatment.
Detection technologies: biosensors, mass spectrometry, DNA-based methods, AI for predictive assessment, next-generation sequencing for profiling.

Main Results:

Advanced processing methods alter protein structures and epitopes, reducing allergenicity.
Emerging detection techniques offer high sensitivity and specificity.
AI and next-generation sequencing enable predictive assessment and high-throughput profiling.

Conclusions:

Enhancing detection and prevention measures can reduce fish allergy incidence and risks.
Strategic utilization of nutritional benefits supports safer fish product development.