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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Updated: Feb 28, 2026

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

Staining normalization in histopathology: Method benchmarking using multicenter dataset

Umair Khan¹, Jouni Härkönen², Marjukka Friman²

¹Institute of Biomedicine, University of Turku, Turku, FI-20014, Finland.

Scientific Reports

|February 26, 2026

Summary

No abstract available in PubMed .

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