Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

5.3K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

7.4K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.4K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

4.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

5.9K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.9K
Negative Regulator Molecules01:23

Negative Regulator Molecules

38.7K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.7K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

6.1K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metformin as a Metabolic Reprogramming Interface in Host-Pathogen and Bone Microenvironment Crosstalk: A Dual-Target Strategy Against Antimicrobial Resistance and Osteoporotic Bone Loss.

Antibiotics (Basel, Switzerland)·2026
Same author

<i>In vivo</i> antiulcerogenic effect of <i>morinda elliptica</i> stem extract against ethanol-induced gastric ulcer in rat.

Journal of Asian natural products research·2026
Same author

Adenosine Signaling as a Central Integrative Network in Cellular Stress Responses and a Therapeutically Actionable Target in Human Disease.

Biomolecules·2026
Same author

Repurposing mebendazole to reprogram oncogenic and tumor-suppressor networks: Multi-cancer insights from ENOX2, MMP2, RASSF1A, WFDC10A and METTL7A.

PloS one·2026
Same author

Chronopharmacology-Driven Precision Therapies for Time-Optimized Cardiometabolic Disease Management.

Biology·2026
Same author

Antibody-Drug Conjugates and Beyond: Next-Generation Targeted Therapies for Breast Cancer.

Cancers·2025

Related Experiment Video

Updated: Feb 28, 2026

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.4K

Pimozide Reprograms the Ran GTPase-SCF Axis and Matrix Remodeling Pathways in Breast, Colorectal, and Pancreatic

Hayat Asaad Hameed Al-Ali1, Mohammad El-Tanani2, Shakta Mani Satyam3

  • 1Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman 19328, Jordan.

Cancers
|February 27, 2026
PubMed
Summary

Pimozide, an antipsychotic drug, shows anticancer effects by disrupting key cancer cell processes like transport and protein regulation. This drug repurposing offers a new multi-target strategy for cancer treatment.

Keywords:
FBXW10Ran GTPaseSCF ubiquitin ligasecancer signalingdrug repurposingmatrix metalloproteinase-2molecular dockingpimozideproteostasis

More Related Videos

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

11.6K
Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer
06:49

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer

Published on: February 10, 2023

2.9K

Related Experiment Videos

Last Updated: Feb 28, 2026

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.4K
Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

11.6K
Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer
06:49

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer

Published on: February 10, 2023

2.9K

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Cancer progression involves complex dysregulation of intracellular transport, proteostasis, and extracellular matrix remodeling.
  • Targeting single pathways often leads to tumor resistance, highlighting the need for multi-target agents.
  • Drug repurposing, using existing drugs like pimozide, offers a strategy for developing novel anticancer therapies with known safety profiles.

Purpose of the Study:

  • To investigate the anticancer effects of pimozide in breast, colorectal, and pancreatic cancer models.
  • To determine pimozide's impact on Ran GTPase signaling, Skp1-Cullin-F-box (SCF) ubiquitin ligase components, and matrix metalloproteinase-2 (MMP-2).

Main Methods:

  • Cell viability assessed via MTT assays in multiple cancer cell lines (MDA-MB-231, MCF-7, HT-29, PanC-1).
  • Gene expression analysis (quantitative real-time PCR) for key genes including Ran, MMP2, Cullin1, Rbx1, SKP2, and FBXW10.
  • Molecular docking and MMGBSA analyses to predict binding interactions between pimozide and target proteins.

Main Results:

  • Pimozide demonstrated concentration-dependent cytotoxicity across all tested cancer cell lines.
  • Consistent downregulation of Ran and MMP-2 observed, indicating disruption of nucleocytoplasmic transport and matrix remodeling.
  • In silico analyses revealed strong binding affinity of pimozide to FBXW10, suggesting interference with ubiquitin-mediated proteostasis.

Conclusions:

  • Pimozide exhibits anticancer properties by simultaneously affecting nucleocytoplasmic transport, proteostasis, and matrix remodeling.
  • These findings support the repositioning of pimozide as a potential multi-target anticancer agent.
  • The study provides a mechanistic basis for further clinical investigation of pimozide in cancer therapy.