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In Vitro Model Characterizing Carcinogenic Progression of HPV-Positive Oropharyngeal Cancer
Jesus Avila Tejeda1, Sreejata Chatterjee1, Craig Meyers1
1Department of Cell and Biological Systems, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA.
Cancers
|February 27, 2026
Summary
We developed an in vitro model to study human papillomavirus (HPV) oral cancer progression. This tool aids in early detection and biomarker discovery for HPV16-driven oropharyngeal squamous cell carcinoma (OPSCC).
Area of Science:
- Oncology
- Virology
- Biotechnology
Background:
- Human papillomavirus (HPV), particularly HPV16, is a leading cause of oropharyngeal squamous cell carcinoma (OPSCC).
- HPV-associated OPSCC incidence is rising globally, especially in developed nations, despite available vaccines.
- Early detection of OPSCC is challenging due to a lack of precursor lesions and long latency periods.
Purpose of the Study:
- To develop an in vitro model simulating HPV16 oral cancer progression.
- To overcome limitations in early detection and biomarker discovery for HPV-associated OPSCC.
Main Methods:
- Utilized a three-dimensional organotypic raft culture system.
- Developed an HPV16 oral cancer model using HPV16-transfected human tonsil keratinocytes.
- Simulated progression from precancerous states to malignant phenotypes in vitro.
Main Results:
- Generated stratified and differentiated epithelia mimicking in vivo biochemical and structural changes.
- Enabled monitoring of epithelial differentiation, biochemical shifts, viral genome status, and oncogenic markers.
- Validated the model by aligning expression profiles with clinical data and measuring virologic and tissue markers.
Conclusions:
- The developed in vitro model is a valuable tool for improving early detection strategies for HPV-associated OPSCC.
- This model can aid in evaluating potential clinical biomarkers for enhanced diagnostic precision.
- The research aims to ultimately improve therapeutic outcomes for patients with HPV-associated OPSCC.
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