In Vitro Antimicrobial Potential of Different Platelet Concentrates Against Eight Clinically Relevant Oral...

Area of Science:

• Microbiology
• Biomaterials Science
• Dental Medicine

Background:

• Oral infections pose challenges due to antimicrobial resistance and reduced antibiotic use.
• Platelet concentrates (PRP, PRF, i-PRF) are used in oral surgery for regeneration and immunomodulation.
• Emerging evidence suggests antimicrobial properties of platelet concentrates.

Purpose of the Study:

• To evaluate the in vitro antimicrobial activity of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and injectable PRF (i-PRF).
• To compare the efficacy of these platelet concentrates against key oral microorganisms.

Main Methods:

• PRP, PRF, and i-PRF were prepared from healthy donors.
• Agar diffusion assays were performed against eight oral pathogens (bacteria and Candida albicans).
• Inhibition zones were measured and statistically analyzed, with chlorhexidine and amoxicillin as positive controls.

Main Results:

• All tested platelet concentrates exhibited microorganism-dependent antimicrobial activity.
• Injectable PRF (i-PRF) demonstrated the most potent inhibitory effect across all tested pathogens (p < 0.001).
• i-PRF showed significantly larger inhibition zones against E. faecalis and C. albicans compared to PRP and PRF.

Conclusions:

• Platelet-derived preparations, particularly i-PRF, show promise as adjunctive antimicrobial agents in dentistry.
• Findings support the potential role of i-PRF in managing dental infections.
• Clinical relevance requires further investigation as in vitro results may not directly translate to patient outcomes.