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NMR Profiling of Milk from Treated Dried off Cows
Antonella Caterina Boccia1, Laura Ruth Cagliani1, Dalila Iannone1
1National Research Council, Institute of Chemical Sciences and Technologies "G. Natta" (SCITEC), via A. Corti 12, 20133 Milan, Italy.
Foods (Basel, Switzerland)
|February 27, 2026
Summary
Dairy cow dry-off management strategies did not alter milk metabolite profiles. Physiological stage significantly impacted milk composition, with no adverse effects observed from treatments.
Area of Science:
- Animal Science
- Metabolomics
- Dairy Cattle Management
Background:
- Dry-off and peripartum periods are critical for dairy cow health and milk production.
- Understanding milk metabolite changes during these periods is crucial for optimizing management.
- Current dry-off strategies require evaluation for their impact on milk composition.
Purpose of the Study:
- To investigate the effects of different dry-off management strategies on dairy cow milk metabolite profiles.
- To compare milk composition between pre-dry-off (T0) and post-calving (T1) stages.
- To assess the influence of teat sealant and *Aloe arborescens* supplementation on milk metabolites.
Main Methods:
- Proton Nuclear Magnetic Resonance (1H NMR) spectroscopy was employed for milk metabolite profiling.
- Chemometric analysis was used to analyze and discriminate metabolite patterns.
- Milk samples were collected from three treatment groups (CTR, SIG, ASIG) at two time points (T0 and T1).
Main Results:
- No significant differences in milk metabolite profiles were found among the CTR, SIG, and ASIG treatment groups.
- A clear metabolic distinction was observed between pre-dry-off (T0) and post-calving (T1) milk samples.
- Specific metabolites like choline, butyrate, BCAAs, and CLAs were higher at T0, while saccharides, citrate, and galactose-1-phosphate were higher at T1.
Conclusions:
- The physiological stage of dairy cows (pre-dry-off vs. post-calving) has a greater impact on milk metabolite profiles than the dry-off management strategies tested.
- The evaluated dry-off treatments (teat sealant, *Aloe arborescens*) did not negatively affect milk metabolite composition.
- Further research can focus on the temporal metabolic shifts related to calving and lactation initiation.
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