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Related Experiment Video

Updated: Feb 28, 2026

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
08:55

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Published on: May 18, 2018

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Intestinal Tissue Damage Reduction After Distal Perfusion for Aortic Arch Surgery in a Neonatal Porcine Model.

Kristin Klaeske1, Sabine Meier1, Jana Lammers1

  • 1Department of Cardiac Surgery, Helios Clinic, University of Leipzig, Heart Center Leipzig, 04289 Leipzig, Germany.

Biomedicines
|February 27, 2026
PubMed
Summary

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Selective anterograde cerebral perfusion with distal perfusion (SACP + DP) reduces neonatal intestinal damage during aortic arch surgery. This method preserves gut structural integrity and enhances protective molecular expression compared to standard SACP.

Area of Science:

  • Cardiovascular Surgery
  • Neonatal Physiology
  • Gastrointestinal Research

Background:

  • Aortic arch reconstruction in neonates presents significant surgical challenges and risks of postoperative complications.
  • Assessing and mitigating intestinal damage during these procedures is critical for patient outcomes.
  • This study compares two perfusion techniques: selective anterograde cerebral perfusion (SACP) and SACP with additional distal perfusion (SACP + DP).

Purpose of the Study:

  • To evaluate and compare the impact of SACP and SACP + DP on intestinal damage in a neonatal piglet model undergoing aortic arch surgery.
  • To investigate histopathological changes, intestinal barrier function, and oxidative stress markers in response to different perfusion strategies.

Main Methods:

  • Neonatal piglets (n=18) were subjected to 60 minutes of cardiac arrest using either SACP (n=9) or SACP + DP (n=9), followed by 120 minutes of recovery.
Keywords:
aortic arch surgerycardiopulmonary bypassintestineneonatespediatricselective anterograde cerebral perfusion

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  • Hemodynamic parameters, blood gases, and electrolytes were continuously monitored.
  • Small intestine and colon biopsies were analyzed for histopathology, barrier function (E-cadherin), and oxidative stress markers (DJ-1, Nrf2).

    • Main Results:

    • The SACP + DP group exhibited less intestinal tissue damage and preserved structural integrity compared to the SACP group.
    • Elevated lactate and reduced pH were observed in the SACP group during cardiac arrest, indicating greater physiological stress.
    • The SACP + DP group showed increased mRNA expression of cytoprotective molecules (DJ-1, Nrf2) and maintained better colonic mucosal structure.

    Conclusions:

    • Selective anterograde cerebral perfusion with distal perfusion (SACP + DP) demonstrates superior protection against intestinal damage during neonatal aortic arch surgery.
    • SACP + DP promotes upregulation of cytoprotective and anti-oxidative stress mechanisms, contributing to reduced intestinal injury.
    • This technique offers a reliable approach to improve postoperative outcomes by minimizing gastrointestinal complications in neonates.