Liquid Chromatography-Mass Spectrometry-Based Molecular Profiling of Vertigoheel

Area of Science:

• Pharmacology
• Analytical Chemistry
• Natural Product Chemistry

Background:

• Vertigoheel is a multicomponent product for vertigo and dizziness.
• Its chemical composition and mechanisms are not fully understood.
• Characterization of active compounds is crucial for understanding efficacy.

Purpose of the Study:

• To profile the chemical composition of Vertigoheel's ingredients and formulations.
• To identify and quantify key marker compounds.
• To establish a robust analytical method for quality control.

Main Methods:

• Ultra-high-performance liquid chromatography coupled to time-of-flight mass spectrometry (UHPLC-ToF-MS) for untargeted profiling.
• Multivariate statistical analysis to identify ingredient-specific metabolites.
• Targeted UHPLC-MS/MS for quantification of specific compounds.

Main Results:

• 68,622 molecular features were detected in ingredient extracts and formulations.
• Ingredient-specific marker compounds were identified (e.g., picrotoxinin, coniine, ambrinol, santalyl phenylacetate).
• Key compounds were quantified at nanogram-per-dose levels with moderate batch variability.

Conclusions:

• Vertigoheel retains characteristic constituents from its natural sources in all formulations.
• The developed analytical protocol allows for absolute quantification of neuroactive molecules.
• This molecular characterization supports further investigation into Vertigoheel's mechanism of action.