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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
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Quantitative Flow Cytometry-Medical Applications with a Focus on Blood Platelets.

Philippe Poncelet1, Thomas Lecompte2, Anne Bauters3

  • 1R and T Department, BioCytex, 13010 Marseille, France.

International Journal of Molecular Sciences
|February 27, 2026
PubMed
Summary
This summary is machine-generated.

Quantitative flow cytometry precisely measures cell-surface molecules, particularly on platelets. This review details its evolution and application in diagnosing platelet disorders and monitoring new therapies.

Keywords:
plateletsquantitative flow cytometrysurface antigens

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Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Flow cytometry quantifies cell-surface molecules using monoclonal antibodies.
  • Its application is extensive in hematology, especially for platelets.
  • Quantitative flow cytometry techniques emerged in the late 20th century.

Purpose of the Study:

  • To review the evolution of quantitative flow cytometry techniques.
  • To highlight specific tools for platelet surface antigen quantitation.
  • To discuss applications in diagnostics and emerging therapies.

Main Methods:

  • Technological advancements in flow cytometry, including calibration beads and immunofluorescence.
  • Development of specific tools for platelet antigen quantification.
  • Review of commercially available kits and their specificities.

Main Results:

  • Platelet antigen quantification aids in diagnosing platelet disorders.
  • Marker expression shows stability in normal individuals but varies with aging and polymorphisms.
  • Emerging targeted therapies may utilize platelet antigen quantification for monitoring.

Conclusions:

  • Quantitative flow cytometry has significantly evolved for cell antigen measurement.
  • Platelet antigen quantification is a valuable diagnostic and monitoring tool.
  • Wider adoption of cell antigen quantification is encouraged.