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Updated: Feb 28, 2026

Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract ECME-based Three-dimensional System

Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract ECME-based Three-dimensional System

Published on: January 8, 2018

Breast-Cancer-Derived Secretomes from MCF-7 Cells Modulate Bacterial Pathogenic Traits.

Suha M Mahmood^1,2, Huda K Al-Nasrallah², Alanoud Aldossry²

¹Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

International Journal of Molecular Sciences

|February 27, 2026

Summary

Breast cancer tumor cells reprogram common bacteria like Pseudomonas aeruginosa and Enterococcus faecalis, altering their structure and antibiotic sensitivity. This breast cancer-microbiome interaction offers new avenues for targeted therapies.

Keywords:

antibiotic susceptibility bacterial virulence biofilm breast cancer conditioned media microbiome

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Last Updated: Feb 28, 2026

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Published on: November 16, 2011

Area of Science:

Microbiology
Oncology
Biotechnology

Background:

Luminal A breast cancer is the most common type globally.
The interaction between breast cancer cells and the microbiome is complex but poorly understood.
Tumor-secreted factors' impact on microbial properties requires further investigation.

Purpose of the Study:

To investigate how luminal A breast cancer cells influence the biological properties of breast-associated bacteria.
To establish an in vitro model simulating the luminal A breast cancer microenvironment.
To explore species-specific tumor-microbiota interactions.

Main Methods:

Utilized conditioned media from MCF-7 (tumor) and MCF-10A (non-tumor) cell lines.
Exposed Pseudomonas aeruginosa, Enterococcus faecalis, and Escherichia coli to conditioned media.
Assessed morphological changes, biofilm formation, antimicrobial susceptibility, and virulence gene expression via qPCR.

Main Results:

MCF-7 conditioned media induced structural alterations in P. aeruginosa and E. faecalis.
Biofilm formation increased in E. coli, while P. aeruginosa and E. faecalis showed varied responses.
Significant changes in antibiotic susceptibility were observed for E. faecalis and P. aeruginosa.
Enrichment of virulence genes in bacteria exposed to tumor cell media was detected.

Conclusions:

Luminal A breast cancer factors can reprogram microbial phenotypes in a species-specific manner.
These findings provide mechanistic insights into breast tumor-microbiome crosstalk.
The study offers a platform for developing microbiome-targeted interventions for breast cancer.