2-Hydroxypropyl-β-Cyclodextrin-Based Complexes Improve Polyphenol Solubility and Bioaccessibility: Evaluation by...

Area of Science:

• Natural Products Chemistry
• Analytical Chemistry
• Pharmacokinetics

Background:

• Propolis polyphenols offer therapeutic benefits but suffer from poor oral bioavailability.
• Developing stable, water-soluble propolis formulations is crucial for oral delivery.

Purpose of the Study:

• To develop and validate an HPLC-DAD method for quantifying propolis polyphenols in HP-β-CD complexes.
• To evaluate the gastrointestinal behavior and bioaccessibility of these complexed polyphenols.

Main Methods:

• A green complexation method using 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) and lyophilization.
• Development and validation of an isocratic HPLC-DAD method for polyphenol quantification.
• Simulated gastrointestinal digestion and intestinal bioaccessibility models.

Main Results:

• The HPLC-DAD method was linear, sensitive, and precise for analyzing polyphenols in HP-β-CD matrices.
• HP-β-CD complexation enhanced polyphenol solubility and gastrointestinal stability.
• Intestinal bioaccessibility varied, but complexation did not enhance intestinal permeation.

Conclusions:

• Validated analytical methods are essential for studying complexed natural products.
• HP-β-CD complexation improves propolis polyphenol solubility and stability but has limitations for intestinal absorption.
• Further strategies are needed to enhance the oral delivery of propolis polyphenols.