Characterization and Insecticidal Efficacy of Green-Synthesized Silver Nanoparticles Against Four Stored Product...

This study aimed to biosynthesize silver nanoparticles (AgNPs) using aqueous leaf extract of Galega officinalis and to evaluate their insecticidal activity against key stored-product pests. AgNP formation was confirmed through UV-vis spectroscopy, which showed a surface plasmon resonance peak at 380 nm. FTIR analysis indicated the presence of plant-derived functional groups likely involved in the reduction and stabilization of Ag⁺ ions. Dynamic light scattering revealed an average hydrodynamic diameter of 25.07 nm, a PDI of 0.39, and a zeta potential of -22 mV, while TEM images showed predominantly spherical and polydisperse particles ranging from 4.3 to 42.4 nm. Insecticidal bioassays performed on Sitophilus granarius, Tribolium confusum, Plodia interpunctella, and Ephestia kuehniella revealed concentration-dependent mortality. The highest mortality rates were recorded at 1000 ppm, reaching 100% in T. confusum, 83.33% in P. interpunctella, and 76.67% in both S. granarius and E. kuehniella. These findings demonstrate the potent insecticidal activity of G. officinalis-mediated AgNPs and support their potential as environmentally friendly alternatives for stored-product pest management, warranting further studies on safety, large-scale synthesis, and integration into pest-control programs.