A Deep Learning-Based Method for Non-Destructive Estimation of Carbonate Carbon Storage in Biogenic Shells on Marine...

Hard-shelled organisms colonizing marine engineering surfaces accumulate carbonate inorganic carbon in their shells, yet quantification typically relies on destructive sampling, hindering long-term monitoring. This study develops a deep learning-based, non-destructive framework to estimate shell carbonate carbon storage from in situ images. Panels of different surface materials were deployed in the nearshore waters of Liuheng Island (Zhoushan) and monitored for five months, yielding 90 panel images from June to October. An improved Mask R-CNN identified barnacles and bivalves and extracted shell dimensions, which were combined with allometric relationships and measured shell carbonate carbon fractions (12.07% for barnacles; 12.14% for bivalves) to estimate carbon storage. Peak colonization occurred on uncoated polyvinyl chloride (PVC) panels in September (~110 individuals per panel), corresponding to 1.061 g carbonate carbon per panel. The model achieved recall/precision of 0.86/0.89 under complex nearshore conditions; image-derived dimensions agreed with manual measurements (R² = 0.95). Allometric models showed R² of 0.82 (barnacles) and 0.90 (bivalves), and panel-scale estimation errors were <15%. The method enables non-destructive quantitative characterization and comparison of shell carbonate carbon storage across materials and exposure conditions for long-term monitoring.