Novel Skin- and Oral-Derived Probiotic Candidates: Functional Evaluation and Application Perspectives

Area of Science:

• Microbiology
• Probiotics
• Skin and Oral Microbiome

Background:

• The skin and oral environments harbor diverse microbial communities with potential health benefits.
• These non-gastrointestinal tract (GIT) microbes may offer properties beyond traditional gut probiotics.

Purpose of the Study:

• To identify and functionally characterize bacterial and yeast isolates from human saliva and skin.
• To evaluate their potential as probiotics for gut and topical applications.

Main Methods:

• Isolates from saliva and skin were identified using API and MALDI-TOF mass spectrometry.
• Functional properties assessed included antimicrobial activity, autoaggregation, antioxidant potential, freeze-drying resistance, and cell adhesion.

Main Results:

• Lacticaseibacillus rhamnosus S3 exhibited significant probiotic potential, including antimicrobial activity against Staphylococcus aureus and tolerance to simulated GIT conditions.
• Limosilactobacillus sp. S1 showed strong antimicrobial activity against Cutibacterium acnes and high adhesion to HaCaT cells, suggesting dermatological use.
• Saccharomyces cerevisiae isolates displayed good autoaggregation and antioxidant capacity but poor freeze-drying resistance.

Conclusions:

• Lacticaseibacillus rhamnosus S3 is a promising candidate for probiotic development.
• Limosilactobacillus sp. S1 holds potential for topical dermatological applications.
• Saccharomyces cerevisiae isolates may have limitations in probiotic formulations due to low freeze-drying stability.